Thrombopoietin Induces Production of Nucleated Thrombocytes from Liver Cells in Xenopus Laevis

Overview

Journal Sci Rep

Specialty Science

Date 2015 Dec 22

PMID 26687619

Citations 1

Authors

Yuta Tanizaki

Megumi Ichisugi

Miyako Obuchi-Shimoji

Takako Ishida-Iwata

Ayaka Tahara-Mogi

Mizue Meguro-Ishikawa

Takashi Kato

Affiliations

Soon will be listed here.

Abstract

The development of mammalian megakaryocytes (MKs) and platelets, which are thought to be absent in non-mammals, is primarily regulated by the thrombopoietin (TPO)/Mpl system. Although non-mammals possess nucleated thrombocytes instead of platelets, the features of nucleated thrombocyte progenitors remain to be clarified. Here, we provide the general features of TPO using Xenopus laevis TPO (xlTPO). Hepatic and splenic cells were cultured in liquid suspension with recombinant xlTPO. These cells differentiated into large, round, polyploid CD41-expressing cells and were classified as X. laevis MKs, comparable to mammalian MKs. The subsequent culture of MKs after removal of xlTPO produced mature, spindle-shaped thrombocytes that were activated by thrombin, thereby altering their morphology. XlTPO induced MKs in cultured hepatic cells for at least three weeks; however, this was not observed in splenic cells; this result demonstrates the origin of early haematopoietic progenitors in the liver rather than the spleen. Additionally, xlTPO enhanced viability of peripheral thrombocytes, indicating the xlTPO-Mpl pathway stimulates anti-apoptotic in peripheral thrombocytes. The development of thrombocytes from MKs via the TPO-Mpl system in X. laevis plays a crucial role in their development from MKs, comparable to mammalian thrombopoiesis. Thus, our results offer insight into the cellular evolution of platelets/MKs in vertebrates. (200/200).

Citing Articles

Flow cytometric analysis of Xenopus laevis and X. tropicalis blood cells using acridine orange.

Sato K, Uehara A, Kinoshita S, Nomura I, Yagi M, Tanizaki Y Sci Rep. 2018; 8(1):16245.

PMID: 30390005 PMC: 6214894. DOI: 10.1038/s41598-018-34631-0.

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