A Systematic Study of Nuclear Interactome of C-terminal Domain Small Phosphatase-like 2 Using Inducible Expression System and Shotgun Proteomics

Overview

Journal BMB Rep

Specialties Biochemistry
Molecular Biology

Date 2015 Dec 18

PMID 26674342

Citations 6

Authors

Nana Kang

JaeHyung Koo

Sen Wang

Sun Jin Hur

Young Yil Bahk

Affiliations

Soon will be listed here.

Abstract

RNA polymerase II C-terminal domain phosphatases are newly emerging family of phosphatases that contain FCPH domain with Mg＋2-binding DXDX(T/V) signature motif. Its subfamily includes small CTD phosphatases (SCPs). Recently, we identified several interacting partners of human SCP1 with appearance of dephosphorylation and O-GlcNAcylation. In this study, using an established cell line with inducible CTDSPL2 protein (a member of the new phosphatase family), proteomic screening was conducted to identify binding partners of CTDSPL2 in nuclear extract through immunoprecipitation of CTDSPL2 with its associated. This approach led to the identification of several interacting partners of CTDSPL2. This will provide a better understanding on CTDSPL2. [BMB Reports 2016; 49(6): 319-324].

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