Actively Replicating Nucleoids Influence Positioning of Division Sites in Escherichia Coli Filaments Forming Cells Lacking DNA

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 Aug 1

PMID 2666394

Citations 78

Authors

E Mulder

C L Woldringh

Affiliations

Soon will be listed here.

Abstract

The positioning of constrictions in Escherichia coli filaments pinching off anucleate cells was analyzed by fluorescence microscopy of dnaX(Ts), dnaX(Ts) sfiA, dnaA46(Ts), gyrA(Am) supF(Ts), and gyrB(Ts) mutants. In filaments with actively replicating nucleoids, constrictions were positioned close to the nucleoid, whereas in nonreplicating filaments, positioning of constrictions within the anucleate region was nearly random. We conclude that constriction positioning depends in an unknown way on nucleoid replication activity.

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