RNA Polymerase II-associated Factor 1 Regulates the Release and Phosphorylation of Paused RNA Polymerase II

Overview

Journal Science

Specialty Science

Date 2015 Dec 15

PMID 26659056

Citations 135

Authors

Ming Yu

Wenjing Yang

Ting Ni

Zhanyun Tang

Tomoyoshi Nakadai

Jun Zhu

Robert G Roeder

Affiliations

Soon will be listed here.

Abstract

Release of promoter-proximal paused RNA polymerase II (Pol II) during early elongation is a critical step in transcriptional regulation in metazoan cells. Paused Pol II release is thought to require the kinase activity of cyclin-dependent kinase 9 (CDK9) for the phosphorylation of DRB sensitivity-inducing factor, negative elongation factor, and C-terminal domain (CTD) serine-2 of Pol II. We found that Pol II-associated factor 1 (PAF1) is a critical regulator of paused Pol II release, that positive transcription elongation factor b (P-TEFb) directly regulates the initial recruitment of PAF1 complex (PAF1C) to genes, and that the subsequent recruitment of CDK12 is dependent on PAF1C. These findings reveal cooperativity among P-TEFb, PAF1C, and CDK12 in pausing release and Pol II CTD phosphorylation.

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