RNAi Mediated Inhibition of Viroid Infection in Transgenic Plants Expressing Viroid-specific Small RNAs Derived from Various Functional Domains

Overview

Journal Sci Rep

Specialty Science

Date 2015 Dec 15

PMID 26656294

Citations 15

Authors

Charith Raj Adkar-Purushothama

Atsushi Kasai

Kohei Sugawara

Hideki Yamamoto

Yuto Yamazaki

Ying-Hong He

Nobuyuki Takada

Hideki Goto

Sahori Shindo

Takeo Harada

Teruo Sano

Affiliations

Soon will be listed here.

Abstract

Previous attempts to develop RNAi-mediated viroid-resistant transgenic plants using nearly full-length Potato spindle tuber viroid (PSTVd) hairpin RNA (hpRNA) were successful; however unusual phenotypes resembling viroid infection occurred. Therefore, in the present work, transgenic Nicotiana benthamiana lines expressing both partial and truncated versions of PSTVd hpRNA were developed. Specifically, seven partial or truncated versions of PSTVd sequences were selected according to the hotspots of both PSTVd-sRNAs and functional domains of the PSTVd. A total of 21 transgenic lines Nicotiana benthamiana were developed under the control of either the CaMV-35S or the CoYMV promoters. All of the transgenic lines established here were monitored for the induction of phenotypic changes, for PSTVd-sRNA expression and for the resistance against PSTVd infection. Additionally, this study demonstrates the use of inverted repeat construct sequences as short as 26- to -49 nucleotides for both the efficient expression of the PSTVd-sRNA and the inhibition of PSTVd infection.

Citing Articles

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Down-regulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection.

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