Deep Sequencing of Hepatitis B Virus Basal Core Promoter and Precore Mutants in HBeAg-positive Chronic Hepatitis B Patients

Overview

Journal Sci Rep

Specialty Science

Date 2015 Dec 10

PMID 26647737

Citations 12

Authors

Linlin Yan

Henghui Zhang

Hui Ma

Di Liu

Wei Li

Yulin Kang

Ruifeng Yang

Jianghua Wang

Gaixia He

Xingwang Xie

Hao Wang

Lai Wei

Zuhong Lu

Qixiang Shao

Hongsong Chen

Affiliations

Soon will be listed here.

Abstract

Mutants in the basal core promoter (BCP) and precore (PC) regions of hepatitis B virus (HBV) genome are associated with the progression of chronic hepatitis B (CHB) infection. However, quasispecies characteristics of naturally occurring mutants in those regions in HBeAg-positive CHB patients has not been well described, partly limited by quantitative assay. This study aimed to develop an Ion Torrent deep sequencing assay to determine BCP and PC mutant percentages in HBeAg-positive CHB patients who were treatment naïve and correlate them with different viral and host factors. Our results showed that Ion Torrent deep sequencing could achieve high accuracy (R(2)>0.99) within a dynamic range between 1% and 100%. Twelve hotspots with prevalence of greater than 20% were observed in EnhII/BCP/PC regions. G1719T, T1753V, A1762T and G1764A were genotype C related. BCP A1762T/G1764A double mutants were generally accompanied with PC 1896 wild type or lower PC G1896A mutant percentage. Lower serum HBeAg and HBsAg levels were associated with higher BCP A1762T/G1764A mutant percentages (≥ 50%). ALT levels were higher in patients with PC G1896A mutant percentage greater than 10%. In conclusion, deep sequencing such as Ion Torrent sequencing could accurately quantify HBV mutants for providing clinical relevant information during HBV infection.

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