Population Genetic Study of 10 Short Tandem Repeat Loci from 600 Domestic Dogs in Korea

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2015 Dec 10

PMID 26645337

Citations 3

Authors

Seo Hyun Moon

Yoon-Jeong Jang

Myun Soo Han

Myung-Haing Cho

Affiliations

Soon will be listed here.

Abstract

Dogs have long shared close relationships with many humans. Due to the large number of dogs in human populations, they are often involved in crimes. Occasionally, canine biological evidence such as saliva, bloodstains and hairs can be found at crime scenes. Accordingly, canine DNA can be used as forensic evidence. The use of short tandem repeat (STR) loci from biological evidence is valuable for forensic investigations. In Korea, canine STR profiling-related crimes are being successfully analyzed, leading to diverse crimes such as animal cruelty, dog-attacks, murder, robbery, and missing and abandoned dogs being solved. However, the probability of random DNA profile matches cannot be analyzed because of a lack of canine STR data. Therefore, in this study, 10 STR loci were analyzed in 600 dogs in Korea (344 dogs belonging to 30 different purebreds and 256 crossbred dogs) to estimate canine forensic genetic parameters. Among purebred dogs, a separate statistical analysis was conducted for five major subgroups, 97 Maltese, 47 Poodles, 31 Shih Tzus, 32 Yorkshire Terriers, and 25 Pomeranians. Allele frequencies, expected (Hexp) and observed heterozygosity (Hobs), fixation index (F), probability of identity (P(ID)), probability of sibling identity (P(ID)sib) and probability of exclusion (PE) were then calculated. The Hexp values ranged from 0.901 (PEZ12) to 0.634 (FHC2079), while the P(ID)sib values were between 0.481 (FHC2079) and 0.304 (PEZ12) and the P(ID)sib was about 3.35 × 10(-)⁵ for the combination of all 10 loci. The results presented herein will strengthen the value of canine DNA to solving dog-related crimes.

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References

Wictum E, Kun T, Lindquist C, Malvick J, Vankan D, Sacks B . Developmental validation of DogFiler, a novel multiplex for canine DNA profiling in forensic casework. Forensic Sci Int Genet. 2012; 7(1):82-91. DOI: 10.1016/j.fsigen.2012.07.001. View

Dayton M, Koskinen M, Tom B, Mattila A, Johnston E, Halverson J . Developmental validation of short tandem repeat reagent kit for forensic DNA profiling of canine biological material. Croat Med J. 2009; 50(3):268-85. PMC: 2702741. DOI: 10.3325/cmj.2009.50.268. View

Zenke P, Egyed B, Zoldag L, Padar Z . Population genetic study in Hungarian canine populations using forensically informative STR loci. Forensic Sci Int Genet. 2010; 5(1):e31-6. DOI: 10.1016/j.fsigen.2010.03.013. View

Calboli F, Sampson J, Fretwell N, Balding D . Population structure and inbreeding from pedigree analysis of purebred dogs. Genetics. 2008; 179(1):593-601. PMC: 2390636. DOI: 10.1534/genetics.107.084954. View

Kanthaswamy S, Tom B, Mattila A, Johnston E, Dayton M, Kinaga J . Canine population data generated from a multiplex STR kit for use in forensic casework. J Forensic Sci. 2009; 54(4):829-40. DOI: 10.1111/j.1556-4029.2009.01080.x. View

Lygo J, Johnson P, Holdaway D, Woodroffe S, Whitaker J, Clayton T . The validation of short tandem repeat (STR) loci for use in forensic casework. Int J Legal Med. 1994; 107(2):77-89. DOI: 10.1007/BF01225493. View

Berger B, Berger C, Hecht W, Hellmann A, Rohleder U, Schleenbecker U . Validation of two canine STR multiplex-assays following the ISFG recommendations for non-human DNA analysis. Forensic Sci Int Genet. 2013; 8(1):90-100. DOI: 10.1016/j.fsigen.2013.07.002. View

Ogden R, Mellanby R, Clements D, Gow A, Powell R, McEwing R . Genetic data from 15 STR loci for forensic individual identification and parentage analyses in UK domestic dogs (Canis lupus familiaris). Forensic Sci Int Genet. 2011; 6(2):e63-5. DOI: 10.1016/j.fsigen.2011.04.015. View

Binns M, Holmes N, Marti E, Bowen N . Dog parentage testing using canine microsatellites. J Small Anim Pract. 1995; 36(11):493-7. DOI: 10.1111/j.1748-5827.1995.tb02791.x. View

10.

DeNise S, Johnston E, Halverson J, Marshall K, Rosenfeld D, McKenna S . Power of exclusion for parentage verification and probability of match for identity in American Kennel Club breeds using 17 canine microsatellite markers. Anim Genet. 2004; 35(1):14-7. DOI: 10.1046/j.1365-2052.2003.01074.x. View

11.

Halverson J, Basten C . A PCR multiplex and database for forensic DNA identification of dogs. J Forensic Sci. 2005; 50(2):352-63. View

12.

Eichmann C, Berger B, Steinlechner M, Parson W . Estimating the probability of identity in a random dog population using 15 highly polymorphic canine STR markers. Forensic Sci Int. 2005; 151(1):37-44. DOI: 10.1016/j.forsciint.2004.07.002. View

13.

Halverson J, Basten C . Forensic DNA identification of animal-derived trace evidence: tools for linking victims and suspects. Croat Med J. 2005; 46(4):598-605. View

14.

Waits L, Luikart G, Taberlet P . Estimating the probability of identity among genotypes in natural populations: cautions and guidelines. Mol Ecol. 2001; 10(1):249-56. DOI: 10.1046/j.1365-294x.2001.01185.x. View

15.

Balding D, Nichols R . DNA profile match probability calculation: how to allow for population stratification, relatedness, database selection and single bands. Forensic Sci Int. 1994; 64(2-3):125-40. DOI: 10.1016/0379-0738(94)90222-4. View

16.

Butler J . Genetics and genomics of core short tandem repeat loci used in human identity testing. J Forensic Sci. 2006; 51(2):253-65. DOI: 10.1111/j.1556-4029.2006.00046.x. View