Comparative Transcriptomic Analysis of Silkworm Bmovo-1 and Wild Type Silkworm Ovary

Overview

Journal Sci Rep

Specialty Science

Date 2015 Dec 9

PMID 26643037

Citations 2

Authors

Renyu Xue

Xiaolong Hu

Liyuan Zhu

Guangli Cao

Moli Huang

Gaoxu Xue

Zuowei Song

Jiayu Lu

Xueying Chen

Chengliang Gong

Affiliations

Soon will be listed here.

Abstract

The detailed molecular mechanism of Bmovo-1 regulation of ovary size is unclear. To uncover the mechanism of Bmovo-1 regulation of ovarian development and oogenesis using RNA-Seq, we compared the transcriptomes of wild type (WT) and Bmovo-1-overexpressing silkworm (silkworm(+Bmovo-1)) ovaries. Using a pair-end Illumina Solexa sequencing strategy, 5,296,942 total reads were obtained from silkworm(+Bmovo-1) ovaries and 6,306,078 from WT ovaries. The average read length was about 100 bp. Clean read ratios were 98.79% for silkworm(+Bmovo-1) and 98.87% for WT silkworm ovaries. Comparative transcriptome analysis showed 123 upregulated and 111 downregulated genes in silkworm(+Bmovo-1) ovaries. These differentially expressed genes were enriched in the extracellular and extracellular spaces and involved in metabolism, genetic information processing, environmental information processing, cellular processes and organismal systems. Bmovo-1 overexpression in silkworm ovaries might promote anabolism for ovarian development and oogenesis and oocyte proliferation and transport of nutrients to ovaries by altering nutrient partitioning, which would support ovary development. Excessive consumption of nutrients for ovary development alters nutrient partitioning and deters silk protein synthesis.

Citing Articles

Functional characterization of BmOVOs in silkworm, Bombyx mori.

Zhu M, Hu X, Liang Z, Jiang M, Xue R, Gong Y BMC Genomics. 2019; 20(1):342.

PMID: 31060506 PMC: 6503385. DOI: 10.1186/s12864-019-5697-y.

Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori.

Sun Z, Kumar D, Cao G, Zhu L, Liu B, Zhu M Sci Rep. 2017; 7(1):3349.

PMID: 28611386 PMC: 5469737. DOI: 10.1038/s41598-017-03565-4.

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