Effects of Carbon Nanotube Environmental Dispersion on an Aquatic Invertebrate, Hirudo Medicinalis

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Dec 5

PMID 26636582

Citations 4

Authors

Rossana Girardello

Stefano Tasselli

Nicolo Baranzini

Roberto Valvassori

Magda de Eguileor

Annalisa Grimaldi

Affiliations

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Abstract

The recent widespread applications of nanomaterials, because of their properties, opens new scenarios that affect their dispersal in the environment. In particular multiwall carbon nanotubes (MWCNTs), despite their qualities, seem to be harmful for animals and humans. To evaluate possible toxic effects caused by carbon nanotube environmental dispersion, with regard to aquatic compartment, we proposed as experimental model a freshwater invertebrate: Hirudo medicinalis. In the present study we analyse acute and chronic immune responses over a short (1, 3, 6 and 12 hours) and long time (from 1 to 5 weeks) exposure to MWCNTs by optical, electron and immunohistochemical approaches. In the exposed leeches angiogenesis and fibroplasia accompanied by massive cellular migration occur. Immunocytochemical characterization using specific markers shows that in these inflammatory processes the monocyte-macrophages (CD45+, CD68+) are the most involved cells. These immunocompetent cells are characterized by sequence of events starting from the expression of pro-inflammatory cytokines (in particular IL-18), and amyloidogenensis. Our combined experimental approaches, basing on high sensitive inflammatory response can highlight adverse effects of nanomaterials on aquatic organisms and could be useful to assess the MWCNTs impact on aquatic, terrestrial animal and human health.

Citing Articles

Nanomaterials and Annelid Immunity: A Comparative Survey to Reveal the Common Stress and Defense Responses of Two Sentinel Species to Nanomaterials in the Environment.

Bodo K, Baranzini N, Girardello R, Kokhanyuk B, Nemeth P, Hayashi Y Biology (Basel). 2020; 9(10).

PMID: 32977601 PMC: 7598252. DOI: 10.3390/biology9100307.

The Toxic Truth About Carbon Nanotubes in Water Purification: a Perspective View.

Das R, Leo B, Murphy F Nanoscale Res Lett. 2018; 13(1):183.

PMID: 29915874 PMC: 6005998. DOI: 10.1186/s11671-018-2589-z.

Cellular responses induced by multi-walled carbon nanotubes: in vivo and in vitro studies on the medicinal leech macrophages.

Girardello R, Baranzini N, Tettamanti G, de Eguileor M, Grimaldi A Sci Rep. 2017; 7(1):8871.

PMID: 28827736 PMC: 5566218. DOI: 10.1038/s41598-017-09011-9.

Systemic distribution of single-walled carbon nanotubes in a novel model: alteration of biochemical parameters, metabolic functions, liver accumulation, and inflammation in vivo.

Principi E, Girardello R, Bruno A, Manni I, Gini E, Pagani A Int J Nanomedicine. 2016; 11:4299-316.

PMID: 27621623 PMC: 5012628. DOI: 10.2147/IJN.S109950.

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