A Rapid Passage Through a Two-active-X-chromosome State Accompanies the Switch of Imprinted X-inactivation Patterns in Mouse Trophoblast Stem Cells

Overview

Journal Epigenetics Chromatin

Publisher Biomed Central

Specialties Biochemistry
Genetics

Date 2015 Dec 3

PMID 26628922

Citations 2

Authors

Julie Prudhomme

Agnes Dubois

Pablo Navarro

Danielle Arnaud

Philip Avner

Celine Morey

Affiliations

Soon will be listed here.

Abstract

Background: In female mice, while the presence of two-active X-chromosomes characterises pluripotency, it is not tolerated in most other cellular contexts. In particular, in the trophoblastic lineage, impairment of paternal X (X(P)) inactivation results in placental defects.

Results: Here, we show that Trophoblast Stem (TS) cells can undergo a complete reversal of imprinted X-inactivation without detectable change in cell-type identity. This reversal occurs through a reactivation of the X(P) leading to TS clones showing two active Xs. Intriguingly, within such clones, all the cells rapidly and homogeneously either re-inactivate the X(P) or inactivate, de novo, the X(M).

Conclusion: This secondary non-random inactivation suggests that the two-active-X states in TS and in pluripotent contexts are epigenetically distinct. These observations also reveal a pronounced plasticity of the TS epigenome allowing TS cells to dramatically and accurately reprogram gene expression profiles. This plasticity may serve as a back-up system when X-linked mono-allelic gene expression is perturbed.

Citing Articles

Initiation of X Chromosome Inactivation during Bovine Embryo Development.

Yu B, van Tol H, Stout T, Roelen B Cells. 2020; 9(4).

PMID: 32325818 PMC: 7226380. DOI: 10.3390/cells9041016.

Involvement of X-chromosome Reactivation in Augmenting Cancer Testis Antigens Expression: A Hypothesis.

Liu C, Luo B, Xie X, Liao X, Fu J, Ge Y Curr Med Sci. 2018; 38(1):19-25.

PMID: 30074147 DOI: 10.1007/s11596-018-1842-0.

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