Rickettsia Rickettsii Isolation from Naturally Infected Amblyomma Parvum Ticks by Centrifugation in a 24-well Culture Plate Technique

Overview

Journal Open Vet J

Specialty Veterinary Medicine

Date 2015 Dec 2

PMID 26623321

Citations 8

Authors

K Dzul-Rosado

G Peniche-Lara

R Tello-Martin

J Zavala-Velazquez

R de Campos Pacheco

M B Labruna

E C Sanchez

J Zavala-Castro

Affiliations

Soon will be listed here.

Abstract

Rocky Mountain spotted fever is an acute illness caused by Rickettsia rickettsii (R. rickettsii) and is transmitted by the bite of ticks of the genera Dermacentor, Amblyomma and Rhipicephalus. The illness results in a high mortality rate and may be easily confused with other febrile syndromes. In Yucatan State, Mexico, childhood cases with a high mortality have been reported. In this work we report the isolation of a Mexican R. rickettsii strain from a tick egg mass using an alternative method for Rickettsia isolation with 24-well plates. We also identified a potential vector of R. rickettsii in the southeast of Mexico, which is Amblyomma parvum.

Citing Articles

SFG and TG seropositivity in Humans suspected of TBD in Yucatan, Mexico.

Dzul Rosado K, Dzul Rosado K, Pena Bates C, Pena Bates C, Tello M, Noh-Pech H Epidemiol Infect. 2025; 153:e21.

PMID: 39773301 PMC: 11795441. DOI: 10.1017/S0950268824001894.

Navigating Q fever: Current perspectives and challenges in outbreak preparedness.

Meles D, Khairullah A, Mustofa I, Wurlina W, Akintunde A, Suwasanti N Open Vet J. 2024; 14(10):2509-2524.

PMID: 39545195 PMC: 11560256. DOI: 10.5455/OVJ.2024.v14.i10.2.

Knowledge, Attitudes, and Practices on Rocky Mountain Spotted Fever among Physicians in a Highly Endemic Region-Mexicali, Mexico.

Bestul N, Padilla R, Montano T, Marquez A, Fierro M, Zazueta O Am J Trop Med Hyg. 2022; 107(4):773-779.

PMID: 35995132 PMC: 9651539. DOI: 10.4269/ajtmh.21-1017.

Possible Association between Selected Tick-Borne Pathogen Prevalence and sensu lato Infestation in Dogs from Juarez City (Chihuahua), Northwest Mexico-US Border.

Beristain-Ruiz D, Garza-Hernandez J, Figueroa-Millan J, Lira-Amaya J, Quezada-Casasola A, Ordonez-Lopez S Pathogens. 2022; 11(5).

PMID: 35631073 PMC: 9145599. DOI: 10.3390/pathogens11050552.

An exploratory analysis of demography and movement patterns of dogs: New insights in the ecology of endemic Rocky Mountain-Spotted Fever in Mexicali, Mexico.

Lopez-Perez A, Orozco L, Zazueta O, Fierro M, Gomez P, Foley J PLoS One. 2020; 15(5):e0233567.

PMID: 32437470 PMC: 7241830. DOI: 10.1371/journal.pone.0233567.

References

Arguello A, Hun L, Rivera P, Taylor L . A fatal urban case of rocky mountain spotted fever presenting an eschar in San Jose, Costa Rica. Am J Trop Med Hyg. 2012; 87(2):345-8. PMC: 3414575. DOI: 10.4269/ajtmh.2012.12-0153. View

Webb L, Carl M, Malloy D, Dasch G, Azad A . Detection of murine typhus infection in fleas by using the polymerase chain reaction. J Clin Microbiol. 1990; 28(3):530-4. PMC: 269657. DOI: 10.1128/jcm.28.3.530-534.1990. View

Regnery R, Spruill C, Plikaytis B . Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol. 1991; 173(5):1576-89. PMC: 207306. DOI: 10.1128/jb.173.5.1576-1589.1991. View

Eremeeva M, Zambrano M, Anaya L, Beati L, Karpathy S, Santos-Silva M . Rickettsia rickettsii in Rhipicephalus ticks, Mexicali, Mexico. J Med Entomol. 2011; 48(2):418-21. DOI: 10.1603/me10181. View

Zavala-Castro J, Zavala-Velazquez J, Walker D, Ruiz Arcila E, Laviada-Molina H, Olano J . Fatal human infection with Rickettsia rickettsii, Yucatán, Mexico. Emerg Infect Dis. 2006; 12(4):672-4. PMC: 3294703. DOI: 10.3201/eid1204.051282. View

Zavala-Castro J, Dzul-Rosado K, Leon J, Walker D, Zavala-Velazquez J . An increase in human cases of spotted fever rickettsiosis in Yucatan, Mexico, involving children. Am J Trop Med Hyg. 2008; 79(6):907-10. PMC: 4264352. View

Gimenez D . STAINING RICKETTSIAE IN YOLK-SAC CULTURES. Stain Technol. 1964; 39:135-40. DOI: 10.3109/10520296409061219. View

Favacho A, Rozental T, Calic S, Scofield M, de Lemos E . Fatal Brazilian spotless fever caused by Rickettsia rickettsii in a dark-skinned patient. Rev Soc Bras Med Trop. 2011; 44(3):395-6. DOI: 10.1590/s0037-86822011000300028. View

Angerami R, da Silva A, Nascimento E, Colombo S, Wada M, Dos Santos F . Brazilian spotted fever: two faces of a same disease? A comparative study of clinical aspects between an old and a new endemic area in Brazil. Clin Microbiol Infect. 2009; 15 Suppl 2:207-8. DOI: 10.1111/j.1469-0691.2008.02160.x. View

10.

Quesada M, Sanfeliu I, Cardenosa N, Segura F . Ten years' experience of isolation of Rickettsia spp. from blood samples using the shell-vial cell culture assay. Ann N Y Acad Sci. 2006; 1078:578-81. DOI: 10.1196/annals.1374.115. View

11.

Zavala-Velazquez J, Cardenas-Marrufo M, Vado-Solis I, Cetina-Camara M, Cano-Tur J, Laviada-Molina H . Hemorrhagic pulmonary leptospirosis: three cases from the Yucatan peninsula, Mexico. Rev Soc Bras Med Trop. 2008; 41(4):404-8. DOI: 10.1590/s0037-86822008000400016. View

12.

Peniche-Lara G, Zavala-Velazquez J, Dzul-Rosado K, Walker D, Zavala-Castro J . Simple method to differentiate among Rickettsia species. J Mol Microbiol Biotechnol. 2013; 23(3):203-8. PMC: 4714558. DOI: 10.1159/000348298. View

13.

Treadwell T, Holman R, Clarke M, Krebs J, Paddock C, Childs J . Rocky Mountain spotted fever in the United States, 1993-1996. Am J Trop Med Hyg. 2001; 63(1-2):21-6. DOI: 10.4269/ajtmh.2000.63.21. View

14.

Dahlgren F, Moonesinghe R, McQuiston J . Short report: Race and rickettsiae: a United States perspective. Am J Trop Med Hyg. 2011; 85(6):1124-5. PMC: 3225164. DOI: 10.4269/ajtmh.2011.11-0462. View

15.

Piranda E, Faccini J, Pinter A, Pacheco R, Cancado P, Labruna M . Experimental infection of Rhipicephalus sanguineus ticks with the bacterium Rickettsia rickettsii, using experimentally infected dogs. Vector Borne Zoonotic Dis. 2010; 11(1):29-36. DOI: 10.1089/vbz.2009.0250. View

16.

Soares J, Soares H, Barbieri A, Labruna M . Experimental infection of the tick Amblyomma cajennense, Cayenne tick, with Rickettsia rickettsii, the agent of Rocky Mountain spotted fever. Med Vet Entomol. 2011; 26(2):139-51. DOI: 10.1111/j.1365-2915.2011.00982.x. View

17.

Martinez-Medina M, Alvarez-Hernandez G, Padilla-Zamudioa J, Rojas-Guerra M . [Rocky Mountain spotted fever in children: clinical and epidemiological features]. Gac Med Mex. 2007; 143(2):137-40. View