Effects of Timed Artificial Insemination Following Estrus Synchronization in Postpartum Beef Cattle

Overview

Journal Open Vet J

Specialty Veterinary Medicine

Date 2015 Dec 2

PMID 26623282

Authors

A Malik

H Wahid

Y Rosnina

A Kasim

M Sabri

Affiliations

Soon will be listed here.

Abstract

The objectives of this study were to evaluate estrus response and pregnancy rates resulting from timed artificial insemination (AI) following estrus synchronization using CIDR in postpartum beef cattle. A total of 100 cows were randomly divided into three groups. Groups 1, 2 and 3 were artificially inseminated at 48-50 h (n=30), 53-55 h (n=30) and 58-60 h (n=40) after CIDR removal, respectively. Estrus synchronization was carried out using a CIDR containing 1.38 mg progesterone. All cows were given 2 mg estradiol benzoate, intramuscularly on the day of CIDR insertion (D 0). The CIDR was removed after 8 days and 125 μg of prostaglandin F2α (PGF2α) was injected intramuscularly. One day after CIDR removal all cows were given 1 mg of estradiol benzoate intramuscularly (D 9). Cows were observed visually for estrus after removal of CIDR. Between 30 and 32 days after timed AI, pregnancy was determined using transrectal ultrasonography. The first estrus observation which is approximately 32 h after CIDR removal showed no significant difference (P>0.05) among the three groups. The onset response of estrus after 32 h removal of CIDR was less than 10% in all three groups 6.6% (G1), 6.8% (G2) and 7.3% (G3). Furthermore, percentages of estrus response (D 10) following CIDR removal were 76.6%, 75.0% and 77.5%. The difference between on D 9 and D 10 estrus response were statistically significant (P<0.05). The pregnancy rates were 23.3% (G1), 26.6% (G2) and 37.5% (G3), which were not significant (P>0.05).

References

Schafer D, Bader J, Meyer J, Haden J, Ellersieck M, Lucy M . Comparison of progestin-based protocols to synchronize estrus and ovulation before fixed-time artificial insemination in postpartum beef cows. J Anim Sci. 2007; 85(8):1940-5. DOI: 10.2527/jas.2006-836. View

Son D, Choe C, Cho S, Choi S, Kim H, Hur T . A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows. J Reprod Dev. 2007; 53(6):1313-8. DOI: 10.1262/jrd.19066. View

Maquivar M, Verduzco A, Galina C, Pulido A, Rojas S, Forster K . Relationship among follicular growth, oestrus, time of ovulation, endogenous estradiol 17beta and luteinizing hormone in Bos indicus cows after a synchronization program. Reprod Domest Anim. 2007; 42(6):571-6. DOI: 10.1111/j.1439-0531.2006.00821.x. View

Lamb G, Stevenson J, Kesler D, Garverick H, Brown D, Salfen B . Inclusion of an intravaginal progesterone insert plus GnRH and prostaglandin F2alpha for ovulation control in postpartum suckled beef cows. J Anim Sci. 2001; 79(9):2253-9. DOI: 10.2527/2001.7992253x. View

Kim U, Suh G, Hur T, Kang S, Kang H, Park S . Comparison of two types of CIDR-based timed artificial insemination protocols for repeat breeder dairy cows. J Reprod Dev. 2007; 53(3):639-45. DOI: 10.1262/jrd.18147. View

Todoroki J, Yamakuchi H, Mizoshita K, Kubota N, Tabara N, Noguchi J . Restoring ovulation in beef donor cows with ovarian cysts by progesterone-releasing intravaginal silastic devices. Theriogenology. 2001; 55(9):1919-32. DOI: 10.1016/s0093-691x(01)00533-7. View

Lucy M, Billings H, Butler W, Ehnis L, Fields M, Kesler D . Efficacy of an intravaginal progesterone insert and an injection of PGF2alpha for synchronizing estrus and shortening the interval to pregnancy in postpartum beef cows, peripubertal beef heifers, and dairy heifers. J Anim Sci. 2001; 79(4):982-95. DOI: 10.2527/2001.794982x. View

Houghton P, Lemenager R, Hendrix K, Moss G, Stewart T . Effects of body composition, pre- and postpartum energy intake and stage of production of energy utilization by beef cows. J Anim Sci. 1990; 68(5):1447-56. DOI: 10.2527/1990.6851447x. View

Rasby R, Day M, Johnson S, Kinder J, Lynch J, Short R . Luteal function and estrus in peripubertal beef heifers treated with an intravaginal progesterone releasing device with or without a subsequent injection of estradiol. Theriogenology. 2000; 50(1):55-63. DOI: 10.1016/s0093-691x(98)00113-7. View

10.

Busch D, Schafer D, Wilson D, Mallory D, Leitman N, Haden J . Timing of artificial insemination in postpartum beef cows following administration of the CO-Synch + controlled internal drug-release protocol. J Anim Sci. 2008; 86(7):1519-25. DOI: 10.2527/jas.2008-0925. View

11.

Leitman N, Busch D, Mallory D, Wilson D, Ellersieck M, Smith M . Comparison of long-term CIDR-based protocols to synchronize estrus in beef heifers. Anim Reprod Sci. 2008; 114(4):345-55. DOI: 10.1016/j.anireprosci.2008.10.014. View

12.

Flores R, Looper M, Kreider D, Post N, Rosenkrans Jr C . Estrous behavior and initiation of estrous cycles in postpartum Brahman-influenced cows after treatment with progesterone and prostaglandin F2alpha. J Anim Sci. 2006; 84(7):1916-25. DOI: 10.2527/jas.2005-692. View

13.

Lammoglia M, Short R, Bellows S, Bellows R, MacNeil M, Hafs H . Induced and synchronized estrus in cattle: dose titration of estradiol benzoate in peripubertal heifers and postpartum cows after treatment with an intravaginal progesterone-releasing insert and prostaglandin F2alpha. J Anim Sci. 1998; 76(6):1662-70. DOI: 10.2527/1998.7661662x. View

14.

Rajamahendran R, Robinson J, Desbottes S, Walton J . Temporal relationships among estrus, body temperature, milk yield, progesterone and luteinizing hormone levels, and ovulation in dairy cows. Theriogenology. 1989; 31(6):1173-82. DOI: 10.1016/0093-691x(89)90086-1. View

15.

Zelinski M, Hirota N, Keenan E, Stormshak F . Influence of exogenous estradiol-17 beta on endometrial progesterone and estrogen receptors during the luteal phase of the ovine estrous cycle. Biol Reprod. 1980; 23(4):743-51. DOI: 10.1095/biolreprod23.4.743. View

16.

Molina R, Galina C, Maquivar M, Estrada S, Chavez A, Diaz G . Pregnancy rate in zebu cows with two different postpartum intervals exposed to a two-bull rotational system. Vet Res Commun. 2003; 27(8):671-80. DOI: 10.1023/a:1027303926829. View

17.

Bader J, Kojima F, Schafer D, Stegner J, Ellersieck M, Smith M . A comparison of progestin-based protocols to synchronize ovulation and facilitate fixed-time artificial insemination in postpartum beef cows. J Anim Sci. 2004; 83(1):136-43. DOI: 10.2527/2005.831136x. View

18.

Day M, Burke C, Taufa V, Day A, Macmillan K . The strategic use of estradiol to enhance fertility and submission rates of progestin-based estrus synchronization programs in dairy herds. J Anim Sci. 2000; 78(3):523-9. DOI: 10.2527/2000.783523x. View

19.

Ando T, Kamimura S, Hamana K, Watanabe G, Taya K . GnRH treatment at CIDR insertion influences ovarian follicular dynamics in Japanese black cows. J Vet Med Sci. 2005; 67(3):275-80. DOI: 10.1292/jvms.67.275. View

20.

Larson J, Lamb G, Stevenson J, Johnson S, Day M, Geary T . Synchronization of estrus in suckled beef cows for detected estrus and artificial insemination and timed artificial insemination using gonadotropin-releasing hormone, prostaglandin F2alpha, and progesterone. J Anim Sci. 2006; 84(2):332-42. DOI: 10.2527/2006.842332x. View