Evaluation of Spheroid Head and Neck Squamous Cell Carcinoma Cell Models in Comparison to Monolayer Cultures

Overview

Journal Oncol Lett

Specialty Oncology

Date 2015 Dec 2

PMID 26622664

Citations 22

Authors

Lorenz Kadletz

Gregor Heiduschka

Julian Domayer

Rainer Schmid

Elisabeth Enzenhofer

Dietmar Thurnher

Affiliations

Soon will be listed here.

Abstract

Two-dimensional (2D) monolayer cell culture models are the most common method used to investigate tumor cells . In the few last decades, a multicellular spheroid model has gained attention due to its adjacency to tumors . The aim of the present study was to investigate immunohistochemical differences between these two cell culture systems. The FaDu, CAL27 and SCC25 head and neck squamous cell carcinoma (HNSCC) cell lines were seeded out in monolayer and multicellular spheroids. The FaDu and SCC25 cells were treated with increasing doses of cisplatin and irradiation. CAL27 cells were not used in theproliferation experiments, since the spheroids of CAL27 cells were not able to process the reagent in CCK-8 assays. Furthermore, they were stained to present alterations of the following antigens: Ki-67, vascular endothelial growth factor receptor, epithelial growth factor and survivin. Differences in growth rates and expression patterns were detected in certain HNSCC cell lines. The proliferation rates showed a significant divergence of cells grown in the three-dimensional model compared with cells grown in the 2D model. Overall, multicellular spheroids are a promising method to reproduce the immunohistochemical aspects and characteristics of tumor cells, and may show different response rates to therapeutic options.

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