Serum Anti-inflammatory Cytokines for the Evaluation of Inflammatory Status in Endometriosis

Overview

Journal J Res Med Sci

Specialty General Medicine

Date 2015 Dec 2

PMID 26622256

Citations 12

Authors

Andrei Mihai Malutan

Tudor Drugan

Razvan Ciortea

Radu Florin Mocan-Hognogi

Carmen Bucuri

Maria Patricia Rada

Dan Mihu

Affiliations

Soon will be listed here.

Abstract

Background: Endometriosis is a frequent gynecologic disease with a severe impact on the quality of life in the affected women; its pathogenesis is yet to be fully understood, with an altered immunity as a possible key factor. The present study aimed to investigate the serum anti-inflammatory cytokine profile in the patients with endometriosis compared with the healthy controls.

Materials And Methods: One hundred and sixty women were included, divided into two study groups (Group I - endometriosis; Group 2 - healthy women). We evaluated the serum levels of interleukin-1 receptor antagonist (IL-1Ra), IL-2, IL-2R, IL-4, IL-10, IL-13, and IL-15 with the use of Human multiplex cytokine panels. Statistical analyses (normality distribution analysis, independent t-test, Mann-Whitney U-test) were performed using IBM SPSS software (version 22.0) and GraphPad Prism (version 5.00); receiver operating characteristic curve were used to demonstrate the diagnostic performance of the studied markers.

Results: The mean serum level of IL-1Ra, IL-4, and IL-10 were significantly higher in women with endometriosis compared to women free of disease from the control group (30.155, 138.459, and 1.489, respectively, compared to 14.109, 84.710, and 0.688, respectively; P < 0.001, P < 0.001, and P = 0.002, respectively.). No significant differences in the mean serum levels of IL-2, IL-13, and IL-15 were observed between the studied groups and IL-2R had a very low detection rate.

Conclusion: Endometriosis is associated with elevated levels of anti-inflammatory cytokines, IL-1Ra, IL-4, and IL-10, markers that have a potential role as a prognostic factor for endometriosis.

Citing Articles

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References

Yin H, Wang S, Dai S . [Study on interferon gamma and interleukin-4 contents of plasma and cultured monoclear cell supernatants in patients with endometriosis]. Zhonghua Fu Chan Ke Za Zhi. 2002; 35(6):327-8. View

Ouyang Z, Osuga Y, Hirota Y, Hirata T, Yoshino O, Koga K . Interleukin-4 induces expression of eotaxin in endometriotic stromal cells. Fertil Steril. 2009; 94(1):58-62. DOI: 10.1016/j.fertnstert.2009.01.129. View

Antsiferova Y, Sotnikova N, Posiseeva L, Shor A . Changes in the T-helper cytokine profile and in lymphocyte activation at the systemic and local levels in women with endometriosis. Fertil Steril. 2005; 84(6):1705-11. DOI: 10.1016/j.fertnstert.2005.05.066. View

Hsieh Y, Chang C, Tsai F, Wu J, Shi Y, Tsai H . Polymorphisms for interleukin-1 beta (IL-1 beta)-511 promoter, IL-1 beta exon 5, and IL-1 receptor antagonist: nonassociation with endometriosis. J Assist Reprod Genet. 2001; 18(9):506-11. PMC: 3455728. DOI: 10.1023/a:1016653127262. View

Santulli P, Borghese B, Chouzenoux S, Streuli I, Borderie D, de Ziegler D . Interleukin-19 and interleukin-22 serum levels are decreased in patients with ovarian endometrioma. Fertil Steril. 2012; 99(1):219-226.e2. DOI: 10.1016/j.fertnstert.2012.08.055. View

Sikora J, Anasz-Kondera Z, Mielczarek-Palacz A, Witek A . [Monocyte activity after stimulation by serum of women with endometriosis]. Ginekol Pol. 2008; 78(10):772-6. View

Podgaec S, Dias Junior J, Chapron C, de Oliveira R, Baracat E, Abrao M . Th1 and Th2 ummune responses related to pelvic endometriosis. Rev Assoc Med Bras (1992). 2010; 56(1):92-8. DOI: 10.1590/s0104-42302010000100022. View

Wu M, Ho H . The role of cytokines in endometriosis. Am J Reprod Immunol. 2003; 49(5):285-96. DOI: 10.1034/j.1600-0897.2003.01207.x. View

Sinaii N, Cleary S, Ballweg M, Nieman L, Stratton P . High rates of autoimmune and endocrine disorders, fibromyalgia, chronic fatigue syndrome and atopic diseases among women with endometriosis: a survey analysis. Hum Reprod. 2002; 17(10):2715-24. DOI: 10.1093/humrep/17.10.2715. View

10.

Drosdzol-Cop A, Skrzypulec-Plinta V, Stojko R . Serum and peritoneal fluid immunological markers in adolescent girls with chronic pelvic pain. Obstet Gynecol Surv. 2012; 67(6):374-81. DOI: 10.1097/OGX.0b013e31825cb12b. View

11.

Elshal M, McCoy J . Multiplex bead array assays: performance evaluation and comparison of sensitivity to ELISA. Methods. 2006; 38(4):317-23. PMC: 1534009. DOI: 10.1016/j.ymeth.2005.11.010. View

12.

Bischoff F, Simpson J . Genetics of endometriosis: heritability and candidate genes. Best Pract Res Clin Obstet Gynaecol. 2004; 18(2):219-32. DOI: 10.1016/j.bpobgyn.2004.01.004. View

13.

Ouyang Z, Hirota Y, Osuga Y, Hamasaki K, Hasegawa A, Tajima T . Interleukin-4 stimulates proliferation of endometriotic stromal cells. Am J Pathol. 2008; 173(2):463-9. PMC: 2475783. DOI: 10.2353/ajpath.2008.071044. View

14.

Kondera-Anasz Z, Sikora J, Mielczarek-Palacz A, Jonca M . Concentrations of interleukin (IL)-1alpha, IL-1 soluble receptor type II (IL-1 sRII) and IL-1 receptor antagonist (IL-1 Ra) in the peritoneal fluid and serum of infertile women with endometriosis. Eur J Obstet Gynecol Reprod Biol. 2005; 123(2):198-203. DOI: 10.1016/j.ejogrb.2005.04.019. View

15.

Khurana Hershey G . IL-13 receptors and signaling pathways: an evolving web. J Allergy Clin Immunol. 2003; 111(4):677-90; quiz 691. DOI: 10.1067/mai.2003.1333. View

16.

Mier-Cabrera J, Jimenez-Zamudio L, Garcia-Latorre E, Cruz-Orozco O, Hernandez-Guerrero C . Quantitative and qualitative peritoneal immune profiles, T-cell apoptosis and oxidative stress-associated characteristics in women with minimal and mild endometriosis. BJOG. 2010; 118(1):6-16. DOI: 10.1111/j.1471-0528.2010.02777.x. View

17.

Rogus J, Beck J, Offenbacher S, Huttner K, Iacoviello L, Latella M . IL1B gene promoter haplotype pairs predict clinical levels of interleukin-1beta and C-reactive protein. Hum Genet. 2008; 123(4):387-98. DOI: 10.1007/s00439-008-0488-6. View

18.

Singh H, Sachan R, Goel H, Mittal B . Genetic variants of interleukin-1RN and interleukin-1beta genes and risk of cervical cancer. BJOG. 2008; 115(5):633-8. DOI: 10.1111/j.1471-0528.2007.01655.x. View

19.

Zhang X, Wen J, Deng L, Lin J . Decreased levels of peritoneal interleukin-1 receptor antagonist in patients with endometriosis and disease-related dysmenorrhea. Fertil Steril. 2007; 88(3):594-9. DOI: 10.1016/j.fertnstert.2006.11.155. View

20.

Fan W, Li S, Chen Q, Huang Z, Ma Q, Xiao Z . Association between interleukin-10 promoter polymorphisms and endometriosis: a meta-analysis. Gene. 2012; 515(1):49-55. DOI: 10.1016/j.gene.2012.11.037. View