Sp. Nov., Isolated from Horse Faeces

The taxonomic position of five strains isolated from horse faeces, and which shared identical 16S rRNA gene sequences, were studied. Cells of all isolates are Gram-stain-negative, obligately aerobic and have a rod-shaped appearance. The strains show highest 16S rRNA gene sequence similarities to (98.3 %), (98.0 %), (97.9 %) and (97.9 %). Whole-genome sequencing of strain 114 and phylogeny reconstruction based on a core set of 1061 genes indicated that CIP 107468 was the closest relative among species of the genus for which whole genome sequences are available. The genomic DNA G+C content of strain 114 is 34.9 mol%, which is lower than any other value reported for the genus . The predominant polyamine is 1,3-diaminopropane, which is typical for the genus . The most abundant fatty acids are Cω7 and/or iso-C 2-OH (36 %) and C (28 %). The proportion of Cω9 (7 %) is distinctively low compared to most species of the genus. The major ubiquinone of strain 114 is Q-9. Microscopic studies revealed the presence of pili and the absence of flagella. The capability of all five strains to utilize l-arabinose and gentisate as well as their lack of growth at temperatures of 41 °C and above provide sufficient criteria to distinguish the isolates from all species of the genus with validly published names. Based on these combined data, the five isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 114 ( = DSM 27228 = CCUG 65204).