Extinction of Hemoglobin Inducibility in Friend Erythroleukemia Cells by Fusion with Cytoplasm of Enucleated Mouse Neuroblastoma or Fibroblast Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Apr 1

PMID 266203

Citations 7

Authors

T V Gopalakrishnan

E B Thompson

W F Anderson

Affiliations

Soon will be listed here.

Abstract

Friend mouse erythroleukemia cells (T3c1-2 and its subline 5000) can be induced to synthesize hemoglobin after treatment with 1.5% (vol/vol) dimethylsulfoxide. When these cells are fused with nonerythroid cells (namely, mouse neuroblastoma or L cells) hemoglobin induction is extinguished. In order to determine if the nucleus of the nonerythroid cell is necessary for this extinction, fusions were performed between mouse erythroleukemia cells and enucleated neuroblastoma or L cells. Hemoglobin induction was reduced or eliminated in clones of these hybrids even after 6 months of continuous culture. These results suggest that the cytoplasm of nonerythroid cells contains factor(s) that extinguish hemoglobin inducibility in erythroleukemic cells and that this new phenotype can be inherited.

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