The Role and Mechanism of α-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2015 Nov 27

PMID 26609522

Citations 23

Authors

Tianlei Chen

Huijuan Mao

Cheng Chen

Lin Wu

Ningning Wang

Xiufen Zhao

Jun Qian

Changying Xing

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the role and possible mechanism of α-Klotho in the calcification and the osteogenic transition of cultured VSMCs.

Methods: VSMCs were cultured in vitro and divided into 5 groups, each using a different medium: (1) control; (2) β-GP; (3) β-GP + Klotho; (4) β-GP + LiCl; (5) β-GP + Klotho + LiCl. Calcium deposits were visualized using Alizarin Red S staining. The calcium concentrations were determined by the o-cresolphthalein complexone method. BMP2, Runx2 and β-catenin levels were estimated by western blotting, and the level of α-SMA was determined by using immunofluorescence at day 12.

Results: β-GP induced an increase in the expression of BMP2, Runx2, and β-catenin. The calcium content increased, and the expression of α-SMA decreased. Alizarin Red S staining was positive under the high phosphorus conditions. BMP2, Runx2, and β-catenin levels and the calcium content decreased when the cells were cultured with rmKlotho; however, the levels of each were upregulated after treatment with the LiCl.

Conclusions: Klotho can ameliorate the calcification and osteogenic transition of VSMCs induced by β-GP. The mechanism of Klotho in preventing calcification in VSMCs may be partially mediated by the inhibition of the Wnt/β-catenin signaling pathway.

Citing Articles

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Klotho Ameliorates Vascular Calcification via Promoting Autophagy.

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The controversy of klotho as a potential biomarker in chronic kidney disease.

Yu L, Li S, Sha M, Kong J, Ye J, Liu Q Front Pharmacol. 2022; 13:931746.

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