Fucoidans Disrupt Adherence of Helicobacter Pylori to AGS Cells In Vitro

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2015 Nov 26

PMID 26604968

Citations 9

Authors

Eng-Guan Chua

Phebe Verbrugghe

Timothy T Perkins

Chin-Yen Tay

Affiliations

Soon will be listed here.

Abstract

Fucoidans are complex sulphated polysaccharides derived from abundant and edible marine algae. Helicobacter pylori is a stomach pathogen that persists in the hostile milieu of the human stomach unless treated with antibiotics. This study aims to provide preliminary data to determine, in vitro, if fucoidans can inhibit the growth of H. pylori and its ability to adhere to gastric epithelial cells (AGS). We analysed the activity of three different fucoidan preparations (Fucus A, Fucus B, and Undaria extracts). Bacterial growth was not arrested or inhibited by the fucoidan preparations supplemented into culture media. All fucoidans, when supplemented into tissue culture media at 1000 µg mL(-1), were toxic to AGS cells and reduced the viable cell count significantly. Fucoidan preparations at 100 µg mL(-1) were shown to significantly reduce the number of adherent H. pylori. These in vitro findings provide the basis for further studies on the clinical use of sulphated polysaccharides as complementary therapeutic agents.

Citing Articles

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References

Maruyama H, Tamauchi H, Hashimoto M, Nakano T . Antitumor activity and immune response of Mekabu fucoidan extracted from Sporophyll of Undaria pinnatifida. In Vivo. 2003; 17(3):245-9. View

Park H, Kim G, Nam T, Kim N, Choi Y . Antiproliferative activity of fucoidan was associated with the induction of apoptosis and autophagy in AGS human gastric cancer cells. J Food Sci. 2011; 76(3):T77-83. DOI: 10.1111/j.1750-3841.2011.02099.x. View

Tay C, Windsor H, Thirriot F, Lu W, Conway C, Perkins T . Helicobacter pylori eradication in Western Australia using novel quadruple therapy combinations. Aliment Pharmacol Ther. 2012; 36(11-12):1076-83. DOI: 10.1111/apt.12089. View

Araya N, Takahashi K, Sato T, Nakamura T, Sawa C, Hasegawa D . Fucoidan therapy decreases the proviral load in patients with human T-lymphotropic virus type-1-associated neurological disease. Antivir Ther. 2011; 16(1):89-98. DOI: 10.3851/IMP1699. View

Lee K, Jeong M, Choi S, Na S, Cha J . Synergistic effect of fucoidan with antibiotics against oral pathogenic bacteria. Arch Oral Biol. 2013; 58(5):482-92. DOI: 10.1016/j.archoralbio.2012.11.002. View

Gao Y, Li C, Yin J, Shen J, Wang H, Wu Y . Fucoidan, a sulfated polysaccharide from brown algae, improves cognitive impairment induced by infusion of Aβ peptide in rats. Environ Toxicol Pharmacol. 2012; 33(2):304-11. DOI: 10.1016/j.etap.2011.12.022. View

Malfertheiner P, Megraud F, OMorain C, Atherton J, Axon A, Bazzoli F . Management of Helicobacter pylori infection--the Maastricht IV/ Florence Consensus Report. Gut. 2012; 61(5):646-64. DOI: 10.1136/gutjnl-2012-302084. View

Megraud F . H pylori antibiotic resistance: prevalence, importance, and advances in testing. Gut. 2004; 53(9):1374-84. PMC: 1774187. DOI: 10.1136/gut.2003.022111. View

DODGSON K . Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochem J. 1961; 78:312-9. PMC: 1205268. DOI: 10.1042/bj0780312. View

10.

DODGSON K, Price R . A note on the determination of the ester sulphate content of sulphated polysaccharides. Biochem J. 1962; 84:106-10. PMC: 1243628. DOI: 10.1042/bj0840106. View

11.

Fukahori S, Yano H, Akiba J, Ogasawara S, Momosaki S, Sanada S . Fucoidan, a major component of brown seaweed, prohibits the growth of human cancer cell lines in vitro. Mol Med Rep. 2011; 1(4):537-42. View

12.

Zhuang C, Itoh H, Mizuno T, Ito H . Antitumor active fucoidan from the brown seaweed, umitoranoo (Sargassum thunbergii). Biosci Biotechnol Biochem. 1995; 59(4):563-7. DOI: 10.1271/bbb.59.563. View

13.

Boo H, Hyun J, Kim S, Kang J, Kim M, Kim S . Fucoidan from Undaria pinnatifida induces apoptosis in A549 human lung carcinoma cells. Phytother Res. 2011; 25(7):1082-6. DOI: 10.1002/ptr.3489. View

14.

Marshall B, Windsor H . The relation of Helicobacter pylori to gastric adenocarcinoma and lymphoma: pathophysiology, epidemiology, screening, clinical presentation, treatment, and prevention. Med Clin North Am. 2005; 89(2):313-44, viii. DOI: 10.1016/j.mcna.2004.09.001. View

15.

Pomin V . Fucanomics and galactanomics: marine distribution, medicinal impact, conceptions, and challenges. Mar Drugs. 2012; 10(4):793-811. PMC: 3366676. DOI: 10.3390/md10040793. View

16.

Hirai I, Sasaki T, Kimoto A, Fujimoto S, Moriyama T, Yamamoto Y . Assessment of East Asian-type cagA-positive Helicobacter pylori using stool specimens from asymptomatic healthy Japanese individuals. J Med Microbiol. 2009; 58(Pt 9):1149-1153. DOI: 10.1099/jmm.0.010934-0. View

17.

Graham D, Fischbach L . Helicobacter pylori treatment in the era of increasing antibiotic resistance. Gut. 2010; 59(8):1143-53. DOI: 10.1136/gut.2009.192757. View

18.

Horiki N, Omata F, Uemura M, Suzuki S, Ishii N, Iizuka Y . Annual change of primary resistance to clarithromycin among Helicobacter pylori isolates from 1996 through 2008 in Japan. Helicobacter. 2009; 14(5):86-90. DOI: 10.1111/j.1523-5378.2009.00714.x. View

19.

Szabo S, Kusstatscher S, Sandor Z, Sakoulas G . Molecular and cellular basis of ulcer healing. Scand J Gastroenterol Suppl. 1995; 208:3-8. DOI: 10.3109/00365529509107754. View

20.

Lamoral-Theys D, Pottier L, Dufrasne F, Neve J, Dubois J, Kornienko A . Natural polyphenols that display anticancer properties through inhibition of kinase activity. Curr Med Chem. 2010; 17(9):812-25. DOI: 10.2174/092986710790712183. View