Improved Production of Melanin from Aspergillus Fumigatus AFGRD105 by Optimization of Media Factors

Overview

Journal AMB Express

Date 2015 Nov 25

PMID 26597959

Citations 16

Authors

Nitya Meenakshi Raman

Pooja Harish Shah

Misha Mohan

Suganthi Ramasamy

Affiliations

Soon will be listed here.

Abstract

Melanins are indolic polymers produced by many genera included among plants, animals and microorganisms and targeted mainly for their wide range of applications in cosmetics, agriculture and medicine. An approach to analyse the cumulative effect of parameters for enhanced melanin production was carried out using response surface methodology. In this present study, optimization of media and process parameters for melanin production from Aspergillus fumigatus AFGRD105 (GenBank: JX041523; NFCCI accession number: 3826) was carried out by an initial univariate approach followed by statistical response surface methodology. The univariate approach was used to standardise the parameters that can be used for the 12-run Plackett-Burman design that is used for screening for critical parameters. Further optimization of parameters was analysed using Box-Behnken design. The optimum conditions observed were temperature, moisture and sodium dihydrogen phosphate concentration. The yield of every run of both designs were confirmed to be melanin by laboratory tests of analysis in the presence of acids, base and water. This is the first report confirming an increase in melanin production A. fumigatus AFGRD105 without the addition of costly additives.

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References

Vazquez M, Martin A . Optimization of Phaffia rhodozyma continuous culture through response surface methodology. Biotechnol Bioeng. 1999; 57(3):314-20. View

Chen Y, Deng Y, Wang J, Cai J, Ren G . Characterization of melanin produced by a wild-type strain of Bacillus thuringiensis. J Gen Appl Microbiol. 2005; 50(4):183-8. DOI: 10.2323/jgam.50.183. View

Nikel P, Pettinari M, Mendez B, Galvagno M . Statistical optimization of a culture medium for biomass and poly(3-hydroxybutyrate) production by a recombinant Escherichia coli strain using agroindustrial byproducts. Int Microbiol. 2006; 8(4):243-50. View

Lagunas-Munoz V, Cabrera-Valladares N, Bolivar F, Gosset G, Martinez A . Optimum melanin production using recombinant Escherichia coli. J Appl Microbiol. 2006; 101(5):1002-8. DOI: 10.1111/j.1365-2672.2006.03013.x. View

McGinnis M . Indoor mould development and dispersal. Med Mycol. 2007; 45(1):1-9. DOI: 10.1080/13693780600928495. View

Sangkharak K, Prasertsan P . Optimization of polyhydroxybutyrate production from a wild type and two mutant strains of Rhodobacter sphaeroides using statistical method. J Biotechnol. 2007; 132(3):331-40. DOI: 10.1016/j.jbiotec.2007.07.721. View

Alviano C, Farbiarz S, De Souza W, Angluster J, Travassos L . Characterization of Fonsecaea pedrosoi melanin. J Gen Microbiol. 1991; 137(4):837-44. DOI: 10.1099/00221287-137-4-837. View

Bajaj I, Lele S, Singhal R . A statistical approach to optimization of fermentative production of poly(gamma-glutamic acid) from Bacillus licheniformis NCIM 2324. Bioresour Technol. 2008; 100(2):826-32. DOI: 10.1016/j.biortech.2008.06.047. View

Bajaj I, Singhal R . Enhanced production of poly (gamma-glutamic acid) from Bacillus licheniformis NCIM 2324 by using metabolic precursors. Appl Biochem Biotechnol. 2008; 159(1):133-41. DOI: 10.1007/s12010-008-8427-5. View

10.

Sajjan S, Kulkarni G, Yaligara V, Kyoung L, Karegoudar T . Purification and physiochemical characterization of melanin pigment from Klebsiella sp. GSK. J Microbiol Biotechnol. 2010; 20(11):1513-20. DOI: 10.4014/jmb.1002.02006. View

11.

Yong X, Raza W, Yu G, Ran W, Shen Q, Yang X . Optimization of the production of poly-γ-glutamic acid by Bacillus amyloliquefaciens C1 in solid-state fermentation using dairy manure compost and monosodium glutamate production residues as basic substrates. Bioresour Technol. 2011; 102(16):7548-54. DOI: 10.1016/j.biortech.2011.05.057. View

12.

Surwase S, Jadhav S, Phugare S, Jadhav J . Optimization of melanin production by Brevundimonas sp. SGJ using response surface methodology. 3 Biotech. 2017; 3(3):187-194. PMC: 3646113. DOI: 10.1007/s13205-012-0082-4. View