Effect of Endometrial Injury on Secretion of Endometrial Cytokines and IVF Outcomes in Women with Unexplained Subfertility

Overview

Journal Mediators Inflamm

Publisher Wiley

Specialties Biochemistry
Pathology

Date 2015 Nov 21

PMID 26586929

Citations 11

Authors

Yu Liang

Junyan Han

Chanwei Jia

Yanmin Ma

Yonglian Lan

Ying Li

Shuyu Wang

Affiliations

Soon will be listed here.

Abstract

In order to determine the effect of endometrial injury (EI) on in vitro fertilization (IVF) outcomes in women with unexplained subfertility and explore the relationship between EI and endometrial inflammatory cytokines, 66 women with unexplained subfertility undergoing IVF treatment were recruited. 38 patients in the EI group underwent EI in the mid-luteal phase of the cycle and 28 patients in the non-EI (NEI) group. According to the pregnancy outcome, the NEI and EI groups were divided into NEI-nonpregnant (NEI-NP), NEI-pregnant (NEI-P), EI-NP, and EI-P. All patients underwent aspiration of endometrial secretions immediately before embryo transfer. The concentrations of ten mediators were measured using Milliplex Magnetic Bead assay. The clinical pregnancy was significantly higher in the EI than in the NEI group. The concentrations of interleukin- (IL-) 6, IL-8, IL-12 (p70), IL-13, interferon- (IFN-) γ, monocyte chemotactic protein- (MCP-) 1, and vascular endothelial growth factor (VEGF) were significantly higher in the EI than the NEI group. The expression of IFN-γ and VEGF in the EI-P was significantly increased compared to the EI-NP group. These findings suggest that, in women with unexplained subfertility, endometrial injury might be a potential method to improve clinical pregnancy rates by promoting the expression of IFN-γ and VEGF.

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References

Almog B, Shalom-Paz E, Dufort D, Tulandi T . Promoting implantation by local injury to the endometrium. Fertil Steril. 2010; 94(6):2026-9. DOI: 10.1016/j.fertnstert.2009.12.075. View

Gibreel A, Badawy A, El-Refai W, El-Adawi N . Endometrial scratching to improve pregnancy rate in couples with unexplained subfertility: a randomized controlled trial. J Obstet Gynaecol Res. 2012; 39(3):680-4. DOI: 10.1111/j.1447-0756.2012.02016.x. View

Boomsma C, Kavelaars A, Eijkemans M, Amarouchi K, Teklenburg G, Gutknecht D . Cytokine profiling in endometrial secretions: a non-invasive window on endometrial receptivity. Reprod Biomed Online. 2009; 18(1):85-94. DOI: 10.1016/s1472-6483(10)60429-4. View

Boomsma C, Kavelaars A, Eijkemans M, Fauser B, Heijnen C, Macklon N . Ovarian stimulation for in vitro fertilization alters the intrauterine cytokine, chemokine, and growth factor milieu encountered by the embryo. Fertil Steril. 2009; 94(5):1764-8. DOI: 10.1016/j.fertnstert.2009.10.044. View

Boomsma C, Kavelaars A, Eijkemans M, Lentjes E, Fauser B, Heijnen C . Endometrial secretion analysis identifies a cytokine profile predictive of pregnancy in IVF. Hum Reprod. 2009; 24(6):1427-35. DOI: 10.1093/humrep/dep011. View

Li H, Lee V, Lau E, Yeung W, Ho P, Ng E . Role of baseline antral follicle count and anti-Mullerian hormone in prediction of cumulative live birth in the first in vitro fertilisation cycle: a retrospective cohort analysis. PLoS One. 2013; 8(4):e61095. PMC: 3634063. DOI: 10.1371/journal.pone.0061095. View

Dekel N, Gnainsky Y, Granot I, Mor G . Inflammation and implantation. Am J Reprod Immunol. 2010; 63(1):17-21. PMC: 3025807. DOI: 10.1111/j.1600-0897.2009.00792.x. View

Jasper M, Tremellen K, Robertson S . Primary unexplained infertility is associated with reduced expression of the T-regulatory cell transcription factor Foxp3 in endometrial tissue. Mol Hum Reprod. 2006; 12(5):301-8. DOI: 10.1093/molehr/gal032. View

Lee H, Schlereth S, Park E, Emami-Naeini P, Chauhan S, Dana R . A novel pro-angiogenic function for interferon-γ-secreting natural killer cells. Invest Ophthalmol Vis Sci. 2014; 55(5):2885-92. PMC: 4010366. DOI: 10.1167/iovs.14-14093. View

10.

Trompezinski S, Denis A, Vinche A, Schmitt D, Viac J . IL-4 and interferon-gamma differentially modulate vascular endothelial growth factor release from normal human keratinocytes and fibroblasts. Exp Dermatol. 2002; 11(3):224-31. DOI: 10.1034/j.1600-0625.2002.110305.x. View

11.

Cheong Y, Boomsma C, Heijnen C, Macklon N . Uterine secretomics: a window on the maternal-embryo interface. Fertil Steril. 2013; 99(4):1093-9. DOI: 10.1016/j.fertnstert.2013.01.144. View

12.

Kim M, Park H, Seol J, Jang J, Cho Y, Kim K . VEGF-A regulated by progesterone governs uterine angiogenesis and vascular remodelling during pregnancy. EMBO Mol Med. 2013; 5(9):1415-30. PMC: 3799495. DOI: 10.1002/emmm.201302618. View

13.

ROWE A, Wulff C, Fraser H . Localization of mRNA for vascular endothelial growth factor (VEGF), angiopoietins and their receptors during the peri-implantation period and early pregnancy in marmosets (Callithrix jacchus). Reproduction. 2003; 126(2):227-38. DOI: 10.1530/rep.0.1260227. View

14.

Boroujerdnia M, Nikbakht R . Beta3 integrin expression within uterine endometrium and its relationship with unexplained infertility. Pak J Biol Sci. 2009; 11(21):2495-9. DOI: 10.3923/pjbs.2008.2495.2499. View

15.

Narvekar S, Gupta N, Shetty N, Kottur A, Srinivas M, Rao K . Does local endometrial injury in the nontransfer cycle improve the IVF-ET outcome in the subsequent cycle in patients with previous unsuccessful IVF? A randomized controlled pilot study. J Hum Reprod Sci. 2010; 3(1):15-9. PMC: 2890904. DOI: 10.4103/0974-1208.63116. View

16.

Gnainsky Y, Granot I, Aldo P, Barash A, Or Y, Mor G . Biopsy-induced inflammatory conditions improve endometrial receptivity: the mechanism of action. Reproduction. 2014; 149(1):75-85. DOI: 10.1530/REP-14-0395. View

17.

van der Gaast M, Classen-Linke I, Krusche C, Beier-Hellwig K, Fauser B, Beier H . Impact of ovarian stimulation on mid-luteal endometrial tissue and secretion markers of receptivity. Reprod Biomed Online. 2008; 17(4):553-63. DOI: 10.1016/s1472-6483(10)60244-1. View

18.

Galliano D, Bellver J, Diaz-Garcia C, Simon C, Pellicer A . ART and uterine pathology: how relevant is the maternal side for implantation?. Hum Reprod Update. 2014; 21(1):13-38. DOI: 10.1093/humupd/dmu047. View

19.

Kalma Y, Granot I, Gnainsky Y, Or Y, Czernobilsky B, Dekel N . Endometrial biopsy-induced gene modulation: first evidence for the expression of bladder-transmembranal uroplakin Ib in human endometrium. Fertil Steril. 2008; 91(4):1042-9, 1049.e1-9. DOI: 10.1016/j.fertnstert.2008.01.043. View

20.

Gnainsky Y, Granot I, Aldo P, Barash A, Or Y, Schechtman E . Local injury of the endometrium induces an inflammatory response that promotes successful implantation. Fertil Steril. 2010; 94(6):2030-6. PMC: 3025806. DOI: 10.1016/j.fertnstert.2010.02.022. View