Genetic Interaction of Aspergillus Nidulans GalR, XlnR and AraR in Regulating D-Galactose and L-Arabinose Release and Catabolism Gene Expression

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Nov 19

PMID 26580075

Citations 25

Authors

Joanna E Kowalczyk

Birgit S Gruben

Evy Battaglia

Ad Wiebenga

Eline Majoor

Ronald P de Vries

Affiliations

Soon will be listed here.

Abstract

In Aspergillus nidulans, the xylanolytic regulator XlnR and the arabinanolytic regulator AraR co-regulate pentose catabolism. In nature, the pentose sugars D-xylose and L-arabinose are both main building blocks of the polysaccharide arabinoxylan. In pectin and arabinogalactan, these two monosaccharides are found in combination with D-galactose. GalR, the regulator that responds to the presence of D-galactose, regulates the D-galactose catabolic pathway. In this study we investigated the possible interaction between XlnR, AraR and GalR in pentose and/or D-galactose catabolism in A. nidulans. Growth phenotypes and metabolic gene expression profiles were studied in single, double and triple disruptant A. nidulans strains of the genes encoding these paralogous transcription factors. Our results demonstrate that AraR and XlnR not only control pentose catabolic pathway genes, but also genes of the oxido-reductive D-galactose catabolic pathway. This suggests an interaction between three transcriptional regulators in D-galactose catabolism. Conversely, GalR is not involved in regulation of pentose catabolism, but controls only genes of the oxido-reductive D-galactose catabolic pathway.

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