LPS-Stimulated Human Skin-Derived Stem Cells Enhance Neo-Vascularization During Dermal Regeneration

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Nov 14

PMID 26565617

Citations 1

Authors

Tobias Kisch

Caroline Weber

Daniel H Rapoport

Charli Kruse

Sandra Schumann

Felix H Stang

Frank Siemers

Anna E Matthiessen

Affiliations

Soon will be listed here.

Abstract

High numbers of adult stem cells are still required to improve the formation of new vessels in scaffolds to accelerate dermal regeneration. Recent data indicate a benefit for vascularization capacity by stimulating stem cells with lipopolysaccharide (LPS). In this study, stem cells derived from human skin (SDSC) were activated with LPS and seeded in a commercially available dermal substitute to examine vascularization in vivo. Besides, in vitro assays were performed to evaluate angiogenic factor release and tube formation ability. Results showed that LPS-activated SDSC significantly enhanced vascularization of the scaffolds, compared to unstimulated stem cells in vivo. Further, in vitro assays confirmed higher secretion rates of proangiogenic as well as proinflammatoric factors in the presence of LPS-activated SDSC. Our results suggest that combining activated stem cells and a dermal substitute is a promising option to enhance vascularization in scaffold-mediated dermal regeneration.

Citing Articles

Combination of inductive effect of lipopolysaccharide and in situ mechanical conditioning for forming an autologous vascular graft in vivo.

Chen C, Guo H, Wang Y, Chang H, Pan C, Tuan-Mu H Sci Rep. 2019; 9(1):10616.

PMID: 31337832 PMC: 6650437. DOI: 10.1038/s41598-019-47054-2.

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