Characterization of Escherichia Coli Isolated from Calf Diarrhea in and Around Kombolcha, South Wollo, Amhara Region, Ethiopia

Overview

Journal Trop Anim Health Prod

Publisher Springer

Specialties Tropical Medicine
Veterinary Medicine

Date 2015 Nov 14

PMID 26563273

Citations 11

Authors

Ashenafi Gebregiorgis

Tesfaye Sisay Tessema

Affiliations

Soon will be listed here.

Abstract

This study was carried out from October 2012 to end of February 2013 in and around Kombolcha, Amhara regional state, Ethiopia, using a total of 201 neonatal calves aged 1 day to 4 months and suffering from diarrhea. The objectives of the study were to isolate Escherichia coli from diarrheic calves, and to determine E. coli biotypes and risk factors associated with its isolation. The fecal samples were collected, transported, and processed following standard microbiological procedures. Seventy-four isolates of E. coli were identified. Yellowish diarrhea, younger age, and low-colostrum feeding were significantly associated with rate of E. coli isolation (P < 0.05). Then the 74 isolates of E. coli were biotyped using fermentation of 9 sugars and grouped into 12 biotypes; the most dominant was biotype III (36.8 %). Finally, by comparing with studies elsewhere, from the 12 isolated biotypes, 3 of them were suggested to be enteropathogenic E. coli (EPEC), entherotoxigenic E. coli (ETEC), and adhesion and effacing E. coli (AEEC) pathogenic strains. The present study showed that E. coli accounted for 37 % of calf diarrhea, with very diverse biotypes.

Citing Articles

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Molecular identification of diarrheagenic Escherichia coli pathotypes and their antibiotic resistance patterns among diarrheic children and in contact calves in Bahir Dar city, Northwest Ethiopia.

Belete M, Demlie T, Chekole W, Tessema T PLoS One. 2022; 17(9):e0275229.

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