Studies on Nucleic Acid Reassociation Kinetics: Rate of Hybridization of Excess RNA with DNA, Compared to the Rate of DNA Renaturation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Mar 1

PMID 265546

Citations 13

Authors

G A Galau

R J Britten

E H Davidson

Affiliations

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Abstract

The rate of reaction of double-stranded replicative form (RF) [3H]DNA of bacteriophage phiX174 with excess (+)strand DNA and (+)strand RNA was measured by standard methods of hydroxyapatite chromatography. The reactions follow pseudo-first-order kinetics and the observed rate constant for the RNA-DNA reaction differs less than 25% from that of the DNA-DNA reaction. The pseudo-first-order rate constants are close to the value predicted on the basis of the second-order rate constant measured in the renaturation of the double-stranded phiX RF [3H]DNA.

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References

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