Therapeutic Effects of Late Outgrowth Endothelial Progenitor Cells or Mesenchymal Stem Cells Derived from Human Umbilical Cord Blood on Infarct Repair

Overview

Journal Int J Cardiol

Publisher Elsevier

Specialty Cardiology & Vascular Diseases

Date 2015 Nov 10

PMID 26551883

Citations 27

Authors

Sung-Whan Kim

Hong Lian Jin

Seok-Min Kang

Sinyoung Kim

Kyung-Jong Yoo

Yangsoo Jang

Hyun Ok Kim

Young-Sup Yoon

Affiliations

Soon will be listed here.

Abstract

Background: This study sought to systematically investigate the derivation of late outgrowth endothelial progenitor cells (late EPC) and mesenchymal stem cells (MSC) from umbilical cord blood (UCB) and to examine their therapeutic effects on myocardial infarction (MI).

Methods: The expression of angiogenic genes was determined by qRT-PCR. Myocardial infarction (MI) was induced in rats, and cells were directly transplanted into the border regions of ischemic heart tissue.

Results: Culture of UCB mononuclear cells yielded two distinct types of cells by morphology after 2 weeks in the same culture conditions. These cells were identified as late EPC and MSC, and each was intramyocardially injected into rat hearts after induction of MI. Echocardiography and histologic analyses demonstrated that both EPC and MSC improved cardiac function and enhanced vascularization, although fibrosis was reduced only in the EPC transplanted hearts. Different paracrine factors were enriched in EPC and MSC. However, once injected into the hearts, they induced similar types of paracrine factors in the heart. Transplanted EPC or MSC were mostly localized at the perivascular areas. This study demonstrated that EPC and MSC can be simultaneously derived from UCB under the same initial culture conditions, and that common paracrine factors are involved in the repair of MI.

Conclusion: Late EPC and MSC are effective for infarct repair, apparently mediated through common humoral mechanisms.

Citing Articles

Unraveling the differential mechanisms of revascularization promoted by MSCs & ECFCs from adipose tissue or umbilical cord in a murine model of critical limb-threatening ischemia.

Rojas-Torres M, Beltran-Camacho L, Martinez-Val A, Sanchez-Gomar I, Eslava-Alcon S, Rosal-Vela A J Biomed Sci. 2024; 31(1):71.

PMID: 39004727 PMC: 11247736. DOI: 10.1186/s12929-024-01059-w.

Endothelial progenitor cells for diabetic cardiac and kidney disease.

Raleigh M, Pasricha S, Nauth A, Ward M, Connelly K Stem Cells Transl Med. 2024; 13(7):625-636.

PMID: 38733609 PMC: 11227977. DOI: 10.1093/stcltm/szae025.

New Insights into the Reparative Angiogenesis after Myocardial Infarction.

Martin-Bornez M, Falcon D, Morrugares R, Siegfried G, Khatib A, Rosado J Int J Mol Sci. 2023; 24(15).

PMID: 37569674 PMC: 10418963. DOI: 10.3390/ijms241512298.

A genome-wide association study identified one variant associated with static spatial working memory in Chinese population.

Zhang L, Zhu Z, Yang Q, Zhao J Front Genet. 2022; 13:915275.

PMID: 36176292 PMC: 9514234. DOI: 10.3389/fgene.2022.915275.

Regulation of Endothelial Progenitor Cell Functions in Ischemic Heart Disease: New Therapeutic Targets for Cardiac Remodeling and Repair.

Huang H, Huang W Front Cardiovasc Med. 2022; 9:896782.

PMID: 35677696 PMC: 9167961. DOI: 10.3389/fcvm.2022.896782.

References

Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson S, Li B . Bone marrow cells regenerate infarcted myocardium. Nature. 2001; 410(6829):701-5. DOI: 10.1038/35070587. View

Orlic D, Kajstura J, Chimenti S, Limana F, Jakoniuk I, Quaini F . Mobilized bone marrow cells repair the infarcted heart, improving function and survival. Proc Natl Acad Sci U S A. 2001; 98(18):10344-9. PMC: 56963. DOI: 10.1073/pnas.181177898. View

Rehman J, Li J, Orschell C, March K . Peripheral blood "endothelial progenitor cells" are derived from monocyte/macrophages and secrete angiogenic growth factors. Circulation. 2003; 107(8):1164-9. DOI: 10.1161/01.cir.0000058702.69484.a0. View

Ingram D, Mead L, Tanaka H, Meade V, Fenoglio A, Mortell K . Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood. Blood. 2004; 104(9):2752-60. DOI: 10.1182/blood-2004-04-1396. View

Tsukahara H, Gordienko D, Tonshoff B, Gelato M, Goligorsky M . Direct demonstration of insulin-like growth factor-I-induced nitric oxide production by endothelial cells. Kidney Int. 1994; 45(2):598-604. DOI: 10.1038/ki.1994.78. View

Mayani H, Lansdorp P . Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood. 1994; 83(9):2410-7. View

Buerke M, Murohara T, Skurk C, Nuss C, Tomaselli K, Lefer A . Cardioprotective effect of insulin-like growth factor I in myocardial ischemia followed by reperfusion. Proc Natl Acad Sci U S A. 1995; 92(17):8031-5. PMC: 41280. DOI: 10.1073/pnas.92.17.8031. View

Hur J, Yoon C, Kim H, Choi J, Kang H, Hwang K . Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis. Arterioscler Thromb Vasc Biol. 2003; 24(2):288-93. DOI: 10.1161/01.ATV.0000114236.77009.06. View

Kinnaird T, Stabile E, Burnett M, Lee C, Barr S, Fuchs S . Marrow-derived stromal cells express genes encoding a broad spectrum of arteriogenic cytokines and promote in vitro and in vivo arteriogenesis through paracrine mechanisms. Circ Res. 2004; 94(5):678-85. DOI: 10.1161/01.RES.0000118601.37875.AC. View

10.

Balsam L, Wagers A, Christensen J, Kofidis T, Weissman I, Robbins R . Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium. Nature. 2004; 428(6983):668-73. DOI: 10.1038/nature02460. View

11.

Bjarnegard M, Enge M, Norlin J, Gustafsdottir S, Fredriksson S, Abramsson A . Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities. Development. 2004; 131(8):1847-57. DOI: 10.1242/dev.01080. View

12.

Urbich C, Dimmeler S . Endothelial progenitor cells: characterization and role in vascular biology. Circ Res. 2004; 95(4):343-53. DOI: 10.1161/01.RES.0000137877.89448.78. View

13.

Sandhu R, Teichert-Kuliszewska K, Nag S, Proteau G, Robb M, Campbell A . Reciprocal regulation of angiopoietin-1 and angiopoietin-2 following myocardial infarction in the rat. Cardiovasc Res. 2004; 64(1):115-24. DOI: 10.1016/j.cardiores.2004.05.013. View

14.

Asahara T, Murohara T, Sullivan A, Silver M, van der Zee R, Li T . Isolation of putative progenitor endothelial cells for angiogenesis. Science. 1997; 275(5302):964-7. DOI: 10.1126/science.275.5302.964. View

15.

Maisonpierre P, Suri C, Jones P, Bartunkova S, Wiegand S, Radziejewski C . Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science. 1997; 277(5322):55-60. DOI: 10.1126/science.277.5322.55. View

16.

Hanahan D . Signaling vascular morphogenesis and maintenance. Science. 1997; 277(5322):48-50. DOI: 10.1126/science.277.5322.48. View

17.

Zamzami N, Brenner C, Marzo I, Susin S, Kroemer G . Subcellular and submitochondrial mode of action of Bcl-2-like oncoproteins. Oncogene. 1998; 16(17):2265-82. DOI: 10.1038/sj.onc.1201989. View

18.

Asahara T, Chen D, Takahashi T, Fujikawa K, Kearney M, Magner M . Tie2 receptor ligands, angiopoietin-1 and angiopoietin-2, modulate VEGF-induced postnatal neovascularization. Circ Res. 1998; 83(3):233-40. DOI: 10.1161/01.res.83.3.233. View

19.

Hellstrom M, Kalen M, Lindahl P, Abramsson A, Betsholtz C . Role of PDGF-B and PDGFR-beta in recruitment of vascular smooth muscle cells and pericytes during embryonic blood vessel formation in the mouse. Development. 1999; 126(14):3047-55. DOI: 10.1242/dev.126.14.3047. View

20.

Nagaya N, Fujii T, Iwase T, Ohgushi H, Itoh T, Uematsu M . Intravenous administration of mesenchymal stem cells improves cardiac function in rats with acute myocardial infarction through angiogenesis and myogenesis. Am J Physiol Heart Circ Physiol. 2004; 287(6):H2670-6. DOI: 10.1152/ajpheart.01071.2003. View