VEGFR-1 Activation-induced MMP-9-dependent Invasion in Hepatocellular Carcinoma

Overview

Journal Future Oncol

Specialty Oncology

Date 2015 Nov 10

PMID 26551737

Citations 16

Authors

Tao Li

Yuhua Zhu

Lihui Han

Wanhua Ren

Hui Liu

Chengyong Qin

Affiliations

Soon will be listed here.

Abstract

Aim: VEGFR-1 can promote invasion through epithelial-mesenchymal transition induction in hepatocellular carcinoma (HCC). This study aims to elucidate VEGFR-1 impact on proteolytic enzymes profile involved with invasion.

Materials & Methods: The effect on cell invasion was evaluated by invasive and migration assays with and without VEGFR-1 activation. The mechanism was investigated by real-time PCR, western blot and gelatin zymography using inhibitors for MMP-9. In total, 95 HCC patients were enrolled for its clinical value evaluation.

Results: VEGFR-1 activation induced invasion in HCC cells with an increase in the expression and activity of MMP-9 and Snail. MMP-9 blockage effectively inhibited VEGFR-1-induced invasion. High coexpression of both in HCC predicted a worse clinical outcome.

Conclusion: Data show a novel VEGFR-1 activation-to-MMP-9 mechanism promoting HCC invasion.

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