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Bone Regeneration by Nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) Scaffolds Seeded with Human Umbilical Cord Mesenchymal Stem Cells in the Calvarial Defects of the Nude Mice

Overview

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2015 Nov 10

PMID 26550565

Citations 5

Authors

Authors

Fei Wang

Xiao-Xia Su

Yu-Cheng Guo

Ang Li

Yin-Cheng Zhang

Hong Zhou

Hu Qiao

Li-Min Guan

Min Zou

Xin-Qin Si

Affiliations

Affiliations

Soon will be listed here.

Abstract

In the preliminary study, we have found an excellent osteogenic property of nanohydroxyapatite/chitosan/poly(lactide-co-glycolide) (nHA/CS/PLGA) scaffolds seeded with human umbilical cord mesenchymal stem cells (hUCMSCs) in vitro and subcutaneously in the nude mice. The aim of this study was to further evaluate the osteogenic capacity of nHA/CS/PLGA scaffolds seeded with hUCMSCs in the calvarial defects of the nude mice. Totally 108 nude mice were included and divided into 6 groups: PLGA scaffolds + hUCMSCs; nHA/PLGA scaffolds + hUCMSCs; CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds + hUCMSCs; nHA/CS/PLGA scaffolds without seeding; the control group (no scaffolds) (n = 18). The scaffolds were implanted into the calvarial defects of nude mice. The amount of new bones was evaluated by fluorescence labeling, H&E staining, and Van Gieson staining at 4 and 8 weeks, respectively. The results demonstrated that the amount of new bones was significantly increased in the group of nHA/CS/PLGA scaffolds seeded with hUCMSCs (p < 0.01). On the basis of previous studies in vitro and in subcutaneous implantation of the nude mice, the results revealed that the nHA and CS also enhanced the bone regeneration by nHA/CS/PLGA scaffolds seeded with hUCMSCs in the calvarial defects of the nude mice at early stage.

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References

1.

Zhu L, Wu Y, Jiang H, Liu W, Cao Y, Zhou G . Engineered cartilage with internal porous high-density polyethylene support from bone marrow stromal cells: A preliminary study in nude mice. Br J Oral Maxillofac Surg. 2009; 48(6):462-5. DOI: 10.1016/j.bjoms.2009.08.012. View

2.

Lee J, Baek S, Venkatesan J, Bhatnagar I, Chang H, Kim H . In vivo study of chitosan-natural nano hydroxyapatite scaffolds for bone tissue regeneration. Int J Biol Macromol. 2014; 67:360-6. DOI: 10.1016/j.ijbiomac.2014.03.053. View

3.

Ford Versypt A, Pack D, Braatz R . Mathematical modeling of drug delivery from autocatalytically degradable PLGA microspheres--a review. J Control Release. 2012; 165(1):29-37. PMC: 3518665. DOI: 10.1016/j.jconrel.2012.10.015. View

4.

Wang F, Zhang Y, Zhou H, Guo Y, Su X . Evaluation of in vitro and in vivo osteogenic differentiation of nano-hydroxyapatite/chitosan/poly(lactide-co-glycolide) scaffolds with human umbilical cord mesenchymal stem cells. J Biomed Mater Res A. 2013; 102(3):760-8. DOI: 10.1002/jbm.a.34747. View

5.

Parent M, Nouvel C, Koerber M, Sapin A, Maincent P, Boudier A . PLGA in situ implants formed by phase inversion: critical physicochemical parameters to modulate drug release. J Control Release. 2013; 172(1):292-304. DOI: 10.1016/j.jconrel.2013.08.024. View

6.

Zhao C, Tan A, Pastorin G, Ho H . Nanomaterial scaffolds for stem cell proliferation and differentiation in tissue engineering. Biotechnol Adv. 2012; 31(5):654-68. DOI: 10.1016/j.biotechadv.2012.08.001. View

7.

Fallahiarezoudar E, Ahmadipourroudposht M, Idris A, Yusof N . A review of: application of synthetic scaffold in tissue engineering heart valves. Mater Sci Eng C Mater Biol Appl. 2015; 48:556-65. DOI: 10.1016/j.msec.2014.12.016. View

8.

Wang S, Zhang Z, Xia L, Zhao J, Sun X, Zhang X . Systematic evaluation of a tissue-engineered bone for maxillary sinus augmentation in large animal canine model. Bone. 2009; 46(1):91-100. DOI: 10.1016/j.bone.2009.09.008. View

9.

Shakir M, Jolly R, Khan M, Iram N, Khan H . Nano-hydroxyapatite/chitosan-starch nanocomposite as a novel bone construct: Synthesis and in vitro studies. Int J Biol Macromol. 2015; 80:282-92. DOI: 10.1016/j.ijbiomac.2015.05.009. View

10.

Balasundaram G, Sato M, Webster T . Using hydroxyapatite nanoparticles and decreased crystallinity to promote osteoblast adhesion similar to functionalizing with RGD. Biomaterials. 2006; 27(14):2798-805. DOI: 10.1016/j.biomaterials.2005.12.008. View

11.

Ramier J, Bouderlique T, Stoilova O, Manolova N, Rashkov I, Langlois V . Biocomposite scaffolds based on electrospun poly(3-hydroxybutyrate) nanofibers and electrosprayed hydroxyapatite nanoparticles for bone tissue engineering applications. Mater Sci Eng C Mater Biol Appl. 2014; 38:161-9. DOI: 10.1016/j.msec.2014.01.046. View

12.

Lahiri D, Ghosh S, Agarwal A . Carbon nanotube reinforced hydroxyapatite composite for orthopedic application: A review. Mater Sci Eng C Mater Biol Appl. 2021; 32(7):1727-1758. DOI: 10.1016/j.msec.2012.05.010. View

13.

Gao Z, Chen F, Zhang J, He L, Cheng X, Ma Q . Vitalisation of tubular coral scaffolds with cell sheets for regeneration of long bones: a preliminary study in nude mice. Br J Oral Maxillofac Surg. 2008; 47(2):116-22. DOI: 10.1016/j.bjoms.2008.07.199. View

14.

Chen W, Liu J, Manuchehrabadi N, Weir M, Zhu Z, Xu H . Umbilical cord and bone marrow mesenchymal stem cell seeding on macroporous calcium phosphate for bone regeneration in rat cranial defects. Biomaterials. 2013; 34(38):9917-25. PMC: 4023544. DOI: 10.1016/j.biomaterials.2013.09.002. View

15.

Gu Y, Huang W, Rahaman M, Day D . Bone regeneration in rat calvarial defects implanted with fibrous scaffolds composed of a mixture of silicate and borate bioactive glasses. Acta Biomater. 2013; 9(11):9126-36. DOI: 10.1016/j.actbio.2013.06.039. View

16.

Lastra M, Molinuevo M, Giussi J, Allegretti P, Blaszczyk-Lezak I, Mijangos C . Tautomerizable β-ketonitrile copolymers for bone tissue engineering: Studies of biocompatibility and cytotoxicity. Mater Sci Eng C Mater Biol Appl. 2015; 51:256-62. DOI: 10.1016/j.msec.2015.03.008. View

17.

Sainitya R, Sriram M, Kalyanaraman V, Dhivya S, Saravanan S, Vairamani M . Scaffolds containing chitosan/carboxymethyl cellulose/mesoporous wollastonite for bone tissue engineering. Int J Biol Macromol. 2015; 80:481-8. DOI: 10.1016/j.ijbiomac.2015.07.016. View

18.

Li X, Nan K, Shi S, Chen H . Preparation and characterization of nano-hydroxyapatite/chitosan cross-linking composite membrane intended for tissue engineering. Int J Biol Macromol. 2011; 50(1):43-9. DOI: 10.1016/j.ijbiomac.2011.09.021. View

19.

Chakravarthi S, Robinson D . Enhanced cellular association of paclitaxel delivered in chitosan-PLGA particles. Int J Pharm. 2011; 409(1-2):111-20. DOI: 10.1016/j.ijpharm.2011.02.034. View

20.

Danhier F, Ansorena E, Silva J, Coco R, Le Breton A, Preat V . PLGA-based nanoparticles: an overview of biomedical applications. J Control Release. 2012; 161(2):505-22. DOI: 10.1016/j.jconrel.2012.01.043. View