Single-cell Transcriptomics Reveals Receptor Transformations During Olfactory Neurogenesis

Overview

Journal Science

Specialty Science

Date 2015 Nov 7

PMID 26541607

Citations 117

Authors

Naresh K Hanchate

Kunio Kondoh

Zhonghua Lu

Donghui Kuang

Xiaolan Ye

Xiaojie Qiu

Lior Pachter

Cole Trapnell

Linda B Buck

Affiliations

Soon will be listed here.

Abstract

The sense of smell allows chemicals to be perceived as diverse scents. We used single-neuron RNA sequencing to explore the developmental mechanisms that shape this ability as nasal olfactory neurons mature in mice. Most mature neurons expressed only one of the ~1000 odorant receptor genes (Olfrs) available, and at a high level. However, many immature neurons expressed low levels of multiple Olfrs. Coexpressed Olfrs localized to overlapping zones of the nasal epithelium, suggesting regional biases, but not to single genomic loci. A single immature neuron could express Olfrs from up to seven different chromosomes. The mature state in which expression of Olfr genes is restricted to one per neuron emerges over a developmental progression that appears to be independent of neuronal activity involving sensory transduction molecules.

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