Inhibition of Tribbles Protein-1 Attenuates Radioresistance in Human Glioma Cells

Overview

Journal Sci Rep

Specialty Science

Date 2015 Nov 3

PMID 26521947

Citations 15

Authors

Bo Tang

Wei Wu

Qing Zhang

Yongjin Sun

Yifen Cui

Fei Wu

Xiaowei Wei

Guangying Qi

Xingsi Liang

Fang Tang

Yunqian Li

Wenhai Fan

Affiliations

Soon will be listed here.

Abstract

Radiotherapy is one of the remedies in the treatment of glioma. The radioresistance is a major drawback, of which the mechanism is unclear. Tribble protein and histone deacetylase are involved in the cancer pathogenesis. This study aims to test a hypothesis that the histone deacetylase inhibitors attenuate the radioresistance in human glioma cells. In this study, human glioma cells were cultured. The cells were treated with irradiation with or without a histone deacetylase inhibitor, butyrate. Apoptosis of the glioma cells was assessed by flow cytometry. The results showed that human glioma cells expressed a low level of Trib1, which was significantly up regulated by exposure to small doses (2 Gy/day for 4 days) of irradiation. Trib1-deficient glioma cells showed an enhanced response to irradiation-induced apoptosis. Exposure to small doses of irradiation, Trib1 formed a complex with pHDAC1 (phosphor histone deacetylase-1) to inhibit p53 expression in glioma cells. The presence of HDAC1 inhibitor, butyrate or parthenolide, significantly enforced irradiation-induced glioma cell apoptosis. In conclusion, the Trib1 plays a critical role in the development of radioresistance of glioma cells. The data suggest that inhibition of Trib1 or HDAC1 has the potential to prevent or attenuate the radioresistance.

Citing Articles

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Current Progress in Delineating the Roles of Pseudokinase TRIB1 in Controlling Human Diseases.

Zhang X, Zhang B, Zhang C, Sun G, Sun X J Cancer. 2021; 12(20):6012-6020.

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