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Apigenin, a Potent Suppressor of Dendritic Cell Maturation and Migration, Protects Against Collagen-induced Arthritis

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Journal J Cell Mol Med
Date 2015 Oct 31
PMID 26515512
Citations 27
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Abstract

This study aimed to investigate whether apigenin (API) suppresses arthritis development through the modulation of dendritic cell functions. Bone marrow-derived dendritic cells (BMDCs) were stimulated in vitro with lipopolysaccharide (LPS) and treated with API for 24 hrs; DC functions, including phenotype expressions, cytokine secretion, phagocytosis and chemotaxis, were then investigated. The effects of API on collagen-induced arthritis (CIA) were examined in vivo, and purified DCs from the lymph nodes (LNs) of API-treated CIA mice were analysed for phenotypes and subsets. In in vitro, API efficiently restrained the phenotypic and functional maturation of LPS-stimulated BMDCs while maintaining phagocytotic capabilities. Moreover, API inhibited the chemotactic responses of LPS-stimulated BMDCs, which may be related to the depressive effect on chemokine receptor 4 (CXCR4). In in vivo, API treatment delayed the onset and reduced the severity of arthritis in CIA mice, and diminished secretion of pro-inflammatory cytokines in the serum and supernatants from the LN cells of the CIA mice. Similar to the in vitro findings, the API-treated mice exhibited reduced expression of co-stimulatory molecules and major histocompatibility complex II on DCs. Furthermore, API treatment strongly down-regulated the number of Langerhans cells, but not plasmacytoid DCs (pDCs) in LNs, which may be related to the depressive effect of API on the expression of CXCR4 on DCs of peripheral blood. These data provide new insight into the mechanism of action of API on arthritis and indicate that the inhibition of maturation and migration of DCs by API may contribute to its immunosuppressive effects.

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References
1.
Thomas R, Davis L, Lipsky P . Rheumatoid synovium is enriched in mature antigen-presenting dendritic cells. J Immunol. 1994; 152(5):2613-23. View

2.
Mounho B, Thrall B . The extracellular signal-regulated kinase pathway contributes to mitogenic and antiapoptotic effects of peroxisome proliferators in vitro. Toxicol Appl Pharmacol. 1999; 159(2):125-33. DOI: 10.1006/taap.1999.8740. View

3.
Firestein G . Evolving concepts of rheumatoid arthritis. Nature. 2003; 423(6937):356-61. DOI: 10.1038/nature01661. View

4.
Lee J, Zhou H, Cho S, Kim Y, Lee Y, Jeong C . Anti-inflammatory mechanisms of apigenin: inhibition of cyclooxygenase-2 expression, adhesion of monocytes to human umbilical vein endothelial cells, and expression of cellular adhesion molecules. Arch Pharm Res. 2007; 30(10):1318-27. DOI: 10.1007/BF02980273. View

5.
Yen J, Khayrullina T, Ganea D . PGE2-induced metalloproteinase-9 is essential for dendritic cell migration. Blood. 2007; 111(1):260-70. PMC: 2200811. DOI: 10.1182/blood-2007-05-090613. View