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Detection of Virus in CSF from the Cases with Meningoencephalitis by Next-generation Sequencing

Overview
Journal J Neurovirol
Publisher Springer
Specialties Microbiology
Neurology
Date 2015 Oct 29
PMID 26506841
Citations 72
Authors
Hongzhi Guan
Ao Shen
Xia Lv
Xunzhe Yang
Haitao Ren
Yanhuan Zhao
Yinxin Zhang
Yanping Gong
Peixiang Ni
Honglong Wu
Yicheng Zhu
Liying Cui
Affiliations
Soon will be listed here.
Abstract

We screened for viral DNA in cerebrospinal fluid samples using next-generation sequencing (NGS) technology to diagnose CNS viral infections. We collected CSF samples from four cases with clinically suspected viral meningoencephalitis. DNA extracted from the samples was analyzed with NGS, and the results were further validated using PCR. Herpes simplex virus 1 (HSV-1) was detected in the CSF of two patients, HSV-2 and human herpes virus type 3 (HHV-3, VZV) in the CSF of two other patients separately. The number of unique reads of the identified viral genes ranged from 144 to 44205 (93.51 to 99.57%). The coverage of identified viral genes ranged from 12 to 98% with a depth value of 1.1 to 35, respectively. The results were further confirmed using PCR in three cases. The clinical presentation and outcomes of these four cases were consistent with the diagnostic results of NGS. NGS of CSF samples can be used as a diagnostic assay for CNS viral infection. Its further application for "pan-viral" or even "pan-microbial" screening of CSF might influence the diagnosis of CNS infectious diseases.

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