De Novo Assembly and Genome Analyses of the Marine-Derived Scopulariopsis Brevicaulis Strain LF580 Unravels Life-Style Traits and Anticancerous Scopularide Biosynthetic Gene Cluster

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Oct 28

PMID 26505484

Citations 20

Authors

Abhishek Kumar

Bernard Henrissat

Mikko Arvas

Muhammad Fahad Syed

Nils Thieme

J Philipp Benz

Jens Laurids Sorensen

Eric Record

Stefanie Poggeler

Frank Kempken

Affiliations

Soon will be listed here.

Abstract

The marine-derived Scopulariopsis brevicaulis strain LF580 produces scopularides A and B, which have anticancerous properties. We carried out genome sequencing using three next-generation DNA sequencing methods. De novo hybrid assembly yielded 621 scaffolds with a total size of 32.2 Mb and 16298 putative gene models. We identified a large non-ribosomal peptide synthetase gene (nrps1) and supporting pks2 gene in the same biosynthetic gene cluster. This cluster and the genes within the cluster are functionally active as confirmed by RNA-Seq. Characterization of carbohydrate-active enzymes and major facilitator superfamily (MFS)-type transporters lead to postulate S. brevicaulis originated from a soil fungus, which came into contact with the marine sponge Tethya aurantium. This marine sponge seems to provide shelter to this fungus and micro-environment suitable for its survival in the ocean. This study also builds the platform for further investigations of the role of life-style and secondary metabolites from S. brevicaulis.

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