Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2015 Oct 28

PMID 26504472

Citations 56

Authors

Kwang Jin Lee

You Chang Oh

Won Kyung Cho

Jin Yeul Ma

Affiliations

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Abstract

This study investigated the antioxidant activity of one hundred kinds of pure chemical compounds found within a number of natural substances and oriental medicinal herbs (OMH). Three different methods were used to evaluate the antioxidant activity of DPPH radical-scavenging activity, ABTS radical-scavenging activity, and online screening HPLC-ABTS assays. The results indicated that 17 compounds exhibited better inhibitory activity against ABTS radical than DPPH radical. The IC50 rate of a more practical substance is determined, and the ABTS assay IC50 values of gallic acid hydrate, (+)-catechin hydrate, caffeic acid, rutin hydrate, hyperoside, quercetin, and kaempferol compounds were 1.03 ± 0.25, 3.12 ± 0.51, 1.59 ± 0.06, 4.68 ± 1.24, 3.54 ± 0.39, 1.89 ± 0.33, and 3.70 ± 0.15 μg/mL, respectively. The ABTS assay is more sensitive to identifying the antioxidant activity since it has faster reaction kinetics and a heightened response to antioxidants. In addition, there was a very small margin of error between the results of the offline-ABTS assay and those of the online screening HPLC-ABTS assay. We also evaluated the effects of 17 compounds on the NO secretion in LPS-stimulated RAW 264.7 cells and also investigated the cytotoxicity of 17 compounds using a cell counting kit (CCK) in order to determine the optimal concentration that would provide an effective anti-inflammatory action with minimum toxicity. These results will be compiled into a database, and this method can be a powerful preselection tool for compounds intended to be studied for their potential bioactivity and antioxidant activity related to their radical-scavenging capacity.

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References

Karacelik A, Kucuk M, Iskefiyeli Z, Aydemir S, De Smet S, Miserez B . Antioxidant components of Viburnum opulus L. determined by on-line HPLC-UV-ABTS radical scavenging and LC-UV-ESI-MS methods. Food Chem. 2015; 175:106-14. DOI: 10.1016/j.foodchem.2014.11.085. View

Yu T, Lee J, Lee Y, Byeon S, Kim M, Sohn E . In vitro and in vivo anti-inflammatory effects of ethanol extract from Acer tegmentosum. J Ethnopharmacol. 2010; 128(1):139-47. DOI: 10.1016/j.jep.2009.12.042. View

Ishimaru K, Nishikawa K, Omoto T, ASAI I, Yoshihira K, Shimomura K . Two flavone 2'-glucosides from Scutellaria baicalensis. Phytochemistry. 1995; 40(1):279-81. DOI: 10.1016/0031-9422(95)00200-q. View

Stewart A, Mullen W, Crozier A . On-line high-performance liquid chromatography analysis of the antioxidant activity of phenolic compounds in green and black tea. Mol Nutr Food Res. 2004; 49(1):52-60. DOI: 10.1002/mnfr.200400064. View

Park H, Yoon S, Choi J, Lee G, Choi J, Shin C . Anticonvulsant effect of wogonin isolated from Scutellaria baicalensis. Eur J Pharmacol. 2007; 574(2-3):112-9. DOI: 10.1016/j.ejphar.2007.07.011. View

Gao Z, Huang K, Yang X, Xu H . Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochim Biophys Acta. 1999; 1472(3):643-50. DOI: 10.1016/s0304-4165(99)00152-x. View

Hazra B, Das Sarma M, Sanyal U . Separation methods of quinonoid constituents of plants used in Oriental traditional medicines. J Chromatogr B Analyt Technol Biomed Life Sci. 2004; 812(1-2):259-75. DOI: 10.1016/j.jchromb.2004.08.007. View

Adegoke O, Forbes P . Challenges and advances in quantum dot fluorescent probes to detect reactive oxygen and nitrogen species: a review. Anal Chim Acta. 2015; 862:1-13. DOI: 10.1016/j.aca.2014.08.036. View

Song X, Li Y, Shi Y, Jin L, Chen J . Quality control of traditional Chinese medicines: a review. Chin J Nat Med. 2013; 11(6):596-607. DOI: 10.1016/S1875-5364(13)60069-2. View

10.

Choi H, Kang O, Park P, Chae H, Oh Y, Lee Y . Mume Fructus water extract inhibits pro-inflammatory mediators in lipopolysaccharide-stimulated macrophages. J Med Food. 2007; 10(3):460-6. DOI: 10.1089/jmf.2006.198. View

11.

Lee K, Song N, Oh Y, Cho W, Ma J . Isolation and Bioactivity Analysis of Ethyl Acetate Extract from Acer tegmentosum Using In Vitro Assay and On-Line Screening HPLC-ABTS(+) System. J Anal Methods Chem. 2014; 2014:150509. PMC: 4216704. DOI: 10.1155/2014/150509. View

12.

Melo P, Massarioli A, Denny C, dos Santos L, Franchin M, Pereira G . Winery by-products: extraction optimization, phenolic composition and cytotoxic evaluation to act as a new source of scavenging of reactive oxygen species. Food Chem. 2015; 181:160-9. DOI: 10.1016/j.foodchem.2015.02.087. View

13.

Kim K, Lee S, Lee Y, Jung S, Park Y, Shin K . Anti-oxidant activities of the extracts from the herbs of Artemisia apiacea. J Ethnopharmacol. 2003; 85(1):69-72. DOI: 10.1016/s0378-8741(02)00338-0. View