Effects of SOX2 on Proliferation, Migration and Adhesion of Human Dental Pulp Stem Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Oct 27

PMID 26496354

Citations 25

Authors

Pengfei Liu

Jinglei Cai

Delu Dong

Yaoyu Chen

Xiaobo Liu

Yi Wang

Yulai Zhou

Affiliations

Soon will be listed here.

Abstract

As a key factor for cell pluripotent and self-renewing phenotypes, SOX2 has attracted scientists' attention gradually in recent years. However, its exact effects in dental pulp stem cells (DPSCs) are still unclear. In this study, we mainly investigated whether SOX2 could affect some biological functions of DPSCs. DPSCs were isolated from the dental pulp of human impacted third molar. SOX2 overexpressing DPSCs (DPSCs-SOX2) were established through retroviral infection. The effect of SOX2 on cell proliferation, migration and adhesion ability was evaluated with CCK-8, trans-well system and fibronectin-induced cell attachment experiment respectively. Whole genome expression of DPSCs-SOX2 was analyzed with RNA microarray. Furthermore, a rescue experiment was performed with SOX2-siRNA in DPSC-SOX2 to confirm the effect of SOX2 overexpression in DPSCs. We found that SOX2 overexpression could result in the enhancement of cell proliferation, migration, and adhesion in DPSCs obviously. RNA microarray analysis indicated that some key genes in the signal pathways associated with cell cycle, migration and adhesion were upregulated in different degree, and the results were further confirmed with qPCR and western-blot. Finally, DPSC-SOX2 transfected with SOX2-siRNA showed a decrease of cell proliferation, migration and adhesion ability, which further confirmed the biological effect of SOX2 in human DPSCs. This study indicated that SOX2 could improve the cell proliferation, migration and adhesion ability of DPSCs through regulating gene expression about cell cycle, migration and adhesion, and provided a novel strategy to develop seed cells with strong proliferation, migration and adhesion ability for tissue engineering.

Citing Articles

Effect of Octamer-Binding Transcription Factor 4 Overexpression on the Neural Induction of Human Dental Pulp Stem Cells.

Gancheva M, Kremer K, Breen J, Arthur A, Hamilton-Bruce A, Thomas P Stem Cell Rev Rep. 2024; 20(3):797-815.

PMID: 38316679 PMC: 10984899. DOI: 10.1007/s12015-024-10678-7.

Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel.

Ivanov A, Kuznetsova A, Popova O, Danilova T, Latyshev A, Yanushevich O Cells. 2023; 12(19).

PMID: 37830549 PMC: 10571948. DOI: 10.3390/cells12192335.

Comparison of and Gene Expression in Leukapheresis-Derived CD34+ Cells before and during Cell Culture.

Swistowska M, Gil-Kulik P, Czop M, Wieczorek K, Macheta A, Petniak A Int J Mol Sci. 2023; 24(4).

PMID: 36835597 PMC: 9962001. DOI: 10.3390/ijms24044186.

Administration of Epidermal Growth Factor (EGF) and Basic Fibroblast Growth Factor (bFGF) to Induce Neural Differentiation of Dental Pulp Stem Cells (DPSC) Isolates.

Lott K, Collier P, Ringor M, Howard K, Kingsley K Biomedicines. 2023; 11(2).

PMID: 36830791 PMC: 9953474. DOI: 10.3390/biomedicines11020255.

Differential Effects of Extracellular Matrix Glycoproteins Fibronectin and Laminin-5 on Dental Pulp Stem Cell Phenotypes and Responsiveness.

Lee H, Bae A, Kim J, Kingsley K J Funct Biomater. 2023; 14(2).

PMID: 36826890 PMC: 9963712. DOI: 10.3390/jfb14020091.

References

Liu T, Wu Y, Guo X, Hui J, Lee E, Lim B . Effects of ectopic Nanog and Oct4 overexpression on mesenchymal stem cells. Stem Cells Dev. 2008; 18(7):1013-22. PMC: 3190294. DOI: 10.1089/scd.2008.0335. View

Paino F, La Noce M, Tirino V, Naddeo P, Desiderio V, Pirozzi G . Histone deacetylase inhibition with valproic acid downregulates osteocalcin gene expression in human dental pulp stem cells and osteoblasts: evidence for HDAC2 involvement. Stem Cells. 2013; 32(1):279-89. PMC: 3963447. DOI: 10.1002/stem.1544. View

DAquino R, De Rosa A, Laino G, Caruso F, Guida L, Rullo R . Human dental pulp stem cells: from biology to clinical applications. J Exp Zool B Mol Dev Evol. 2008; 312B(5):408-15. DOI: 10.1002/jez.b.21263. View

Liu L, Wu L, Wei X, Ling J . Induced overexpression of Oct4A in human dental pulp cells enhances pluripotency and multilineage differentiation capability. Stem Cells Dev. 2014; 24(8):962-72. DOI: 10.1089/scd.2014.0388. View

Qin Z, Li Y, Li Y, Liu G . Tumor Necrosis Factor Alpha Stimulates Proliferation of Dental Pulp Stem Cells via Akt/Glycogen Synthase Kinase-3β/Cyclin D1 Signaling Pathway. J Endod. 2015; 41(7):1066-72. DOI: 10.1016/j.joen.2015.02.020. View

Qin Y, Zhou C, Wang N, Yang H, Gao W . Conversion of Adipose Tissue-Derived Mesenchymal Stem Cells to Neural Stem Cell-Like Cells by a Single Transcription Factor, Sox2. Cell Reprogram. 2015; 17(3):221-6. PMC: 4487254. DOI: 10.1089/cell.2015.0001. View

Liu N, Tian J, Cheng J, Zhang J . Migration of CXCR4 gene-modified bone marrow-derived mesenchymal stem cells to the acute injured kidney. J Cell Biochem. 2013; 114(12):2677-89. DOI: 10.1002/jcb.24615. View

Tamaoki N, Takahashi K, Tanaka T, Ichisaka T, Aoki H, Takeda-Kawaguchi T . Dental pulp cells for induced pluripotent stem cell banking. J Dent Res. 2010; 89(8):773-8. DOI: 10.1177/0022034510366846. View

Liu P, Feng Y, Dong C, Liu D, Wu X, Wu H . Study on therapeutic action of bone marrow derived mesenchymal stem cell combined with vitamin E against acute kidney injury in rats. Life Sci. 2013; 92(14-16):829-37. DOI: 10.1016/j.lfs.2013.02.016. View

10.

Hutz K, Mejias-Luque R, Farsakova K, Ogris M, Krebs S, Anton M . The stem cell factor SOX2 regulates the tumorigenic potential in human gastric cancer cells. Carcinogenesis. 2013; 35(4):942-50. DOI: 10.1093/carcin/bgt410. View

11.

Matsuzawa M, Arai C, Nomura Y, Murata T, Yamakoshi Y, Oida S . Periostin of human periodontal ligament fibroblasts promotes migration of human mesenchymal stem cell through the αvβ3 integrin/FAK/PI3K/Akt pathway. J Periodontal Res. 2015; 50(6):855-63. DOI: 10.1111/jre.12277. View

12.

Trounson A, Thakar R, Lomax G, Gibbons D . Clinical trials for stem cell therapies. BMC Med. 2011; 9:52. PMC: 3098796. DOI: 10.1186/1741-7015-9-52. View

13.

Gronthos S, Mankani M, Brahim J, Robey P, Shi S . Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97(25):13625-30. PMC: 17626. DOI: 10.1073/pnas.240309797. View

14.

Liu P, Feng Y, Dong C, Yang D, Li B, Chen X . Administration of BMSCs with muscone in rats with gentamicin-induced AKI improves their therapeutic efficacy. PLoS One. 2014; 9(5):e97123. PMC: 4019657. DOI: 10.1371/journal.pone.0097123. View

15.

Lin F, Lin P, Zhao D, Chen Y, Xiao L, Qin W . Sox2 targets cyclinE, p27 and survivin to regulate androgen-independent human prostate cancer cell proliferation and apoptosis. Cell Prolif. 2012; 45(3):207-16. PMC: 6495851. DOI: 10.1111/j.1365-2184.2012.00812.x. View

16.

Masui S, Nakatake Y, Toyooka Y, Shimosato D, Yagi R, Takahashi K . Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat Cell Biol. 2007; 9(6):625-35. DOI: 10.1038/ncb1589. View

17.

Pisciotta A, Carnevale G, Meloni S, Riccio M, De Biasi S, Gibellini L . Human dental pulp stem cells (hDPSCs): isolation, enrichment and comparative differentiation of two sub-populations. BMC Dev Biol. 2015; 15:14. PMC: 4377026. DOI: 10.1186/s12861-015-0065-x. View

18.

La Noce M, Paino F, Spina A, Naddeo P, Montella R, Desiderio V . Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. J Dent. 2014; 42(7):761-8. DOI: 10.1016/j.jdent.2014.02.018. View

19.

Gao F, Hu X, Xie X, Liu X, Wang J . Heat shock protein 90 stimulates rat mesenchymal stem cell migration via PI3K/Akt and ERK1/2 pathways. Cell Biochem Biophys. 2014; 71(1):481-9. DOI: 10.1007/s12013-014-0228-6. View

20.

Conde M, Nedel F, Campos V, Smith A, Nor J, Demarco F . Odontoblast RNA stability in different temperature-based protocols for tooth storage. Int Endod J. 2011; 45(3):266-72. DOI: 10.1111/j.1365-2591.2011.01971.x. View