Levels of Dioxin-like PCBs in Low-volume Serum Samples of Male Patients Attending Fertility Clinics

Overview

Journal Environ Sci Pollut Res Int

Publisher Springer

Specialties Environmental Health
Toxicology

Date 2015 Oct 23

PMID 26490925

Citations 1

Authors

Julia Molto

Raiza Paul

Nuria Ortuno

Maria Llanos Medrano

Jon Aizpurua

Maria Jose Gomez-Torres

Affiliations

Soon will be listed here.

Abstract

An accurate and easy method for the extraction, cleanup, and HRGC-HRMS analysis of dioxin-like PCBs (DL-PCBs) in low-volume serum samples (1 mL) was developed. Serum samples were extracted several times using n-hexane and purified by acid washing. Recovery rates of labeled congeners ranged from 70 to 110% and the limits of detection were below 1 pg/g on lipid basis. Although human studies are limited and contradictory, several studies have shown that DL-PCBs can have adverse effects on the male reproductive system. In this way, the present method was applied to 21 serum samples of male patients attending fertility clinics. The total levels obtained for the patients ranged from 6.90 to 84.1 pg WHO-TEQ/g lipid, with a mean value of 20.3 pg WHO-TEQ/g lipid. The predominant PCBs (the sum of PCB 118, 156, and 105) contributed 67% to the mean concentration of total DL-PCBs in the samples analyzed.

Citing Articles

Endocrine-Disrupting Air Pollutants and Their Effects on the Hypothalamus-Pituitary-Gonadal Axis.

Plunk E, Richards S Int J Mol Sci. 2020; 21(23).

PMID: 33276521 PMC: 7731392. DOI: 10.3390/ijms21239191.

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