Correlation Between Cell Migration and Reactive Oxygen Species Under Electric Field Stimulation

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2015 Oct 22

PMID 26487906

Citations 13

Authors

Shang-Ying Wu

Hsien-San Hou

Yung-Shin Sun

Ji-Yen Cheng

Kai-Yin Lo

Affiliations

Soon will be listed here.

Abstract

Cell migration is an essential process involved in the development and maintenance of multicellular organisms. Electric fields (EFs) are one of the many physical and chemical factors known to affect cell migration, a phenomenon termed electrotaxis or galvanotaxis. In this paper, a microfluidics chip was developed to study the migration of cells under different electrical and chemical stimuli. This chip is capable of providing four different strengths of EFs in combination with two different chemicals via one simple set of agar salt bridges and Ag/AgCl electrodes. NIH 3T3 fibroblasts were seeded inside this chip to study their migration and reactive oxygen species (ROS) production in response to different EF strengths and the presence of β-lapachone. We found that both the EF and β-lapachone level increased the cell migration rate and the production of ROS in an EF-strength-dependent manner. A strong linear correlation between the cell migration rate and the amount of intracellular ROS suggests that ROS are an intermediate product by which EF and β-lapachone enhance cell migration. Moreover, an anti-oxidant, α-tocopherol, was found to quench the production of ROS, resulting in a decrease in the migration rate.

Citing Articles

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SCHEEPDOG: Programming Electric Cues to Dynamically Herd Large-Scale Cell Migration.

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Hub Proteins Involved in RAW 264.7 Macrophages Exposed to Direct Current Electric Field.

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Cellular processes involved in lung cancer cells exposed to direct current electric field.

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Biomedical applications of electrical stimulation.

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