Identification of TEL-AML1 (ETV6-RUNX1) Associated DNA and Its Impact on MRNA and Protein Output Using ChIP, MRNA Expression Arrays and SILAC

Overview

Journal Genom Data

Specialty Genetics

Date 2015 Oct 21

PMID 26484077

Citations 2

Authors

Yvonne Linka

Sebastian Ginzel

Arndt Borkhardt

Pablo Landgraf

Affiliations

Soon will be listed here.

Abstract

The contribution of the most common reciprocal translocation in childhood B-cell precursor leukemia t(12;21)(p13;q22) to leukemia development is still under debate. Direct as well as secondary indirect effects of the TEL-AML1 fusion protein are commonly recorded by using cell lines and patient samples, often bearing the TEL-AML1 fusion protein for decades. To identify direct targets of the fusion protein a short-term induction of TEL-AML1 is needed. We here describe in detail the experimental procedure, quality controls and contents of the ChIP, mRNA expression and SILAC datasets associated with the study published by Linka and colleagues in the Blood Cancer Journal [1] utilizing a short term induction of TEL-AML1 in an inducible precursor B-cell line model.

Citing Articles

The correction of ETV6/RUNX1 translocation in acute lymphocytic leukemia cells: a new gene targeting system by homologous recombination mechanism.

Akbari M, Ebrahimabadi S, Golalipour M, Shahbazi M, Farazmandfar T J Appl Genet. 2019; 61(1):67-73.

PMID: 31602594 DOI: 10.1007/s13353-019-00524-9.

Genome wide mapping of ETV6 binding sites in pre-B leukemic cells.

Neveu B, Caron M, Lagace K, Richer C, Sinnett D Sci Rep. 2018; 8(1):15526.

PMID: 30341373 PMC: 6195514. DOI: 10.1038/s41598-018-33947-1.

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