Detection of Hepatitis B Virus Infection: A Systematic Review

Overview

Journal World J Hepatol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2015 Oct 21

PMID 26483870

Citations 11

Authors

Mallika Ghosh

Srijita Nandi

Shrinwanti Dutta

Malay Kumar Saha

Affiliations

Soon will be listed here.

Abstract

Aim: To review published methods for detection of hepatitis B virus (HBV) infection.

Methods: A thorough search on Medline database was conducted to find original articles describing different methods or techniques of detection of HBV, which are published in English in last 10 years. Articles outlining methods of detection of mutants or drug resistance were excluded. Full texts and abstracts (if full text not available) were reviewed thoroughly. Manual search of references of retrieved articles were also done. We extracted data on different samples and techniques of detection of HBV, their sensitivity (Sn), specificity (Sp) and applicability.

Results: A total of 72 studies were reviewed. HBV was detected from dried blood/plasma spots, hepatocytes, ovarian tissue, cerumen, saliva, parotid tissue, renal tissue, oocytes and embryos, cholangiocarcinoma tissue, etc. Sensitivity of dried blood spot for detecting HBV was > 90% in all the studies. In case of seronegative patients, HBV DNA or serological markers have been detected from hepatocytes or renal tissue in many instances. Enzyme linked immunosorbent assay and Chemiluminescent immunoassay (CLIA) are most commonly used serological tests for detection. CLIA systems are also used for quantitation. Molecular techniques are used qualitatively as well as for quantitative detection. Among the molecular techniques version 2.0 of the CobasAmpliprep/CobasTaqMan assay and Abbott's real time polymerase chain reaction kit were found to be most sensitive with a lower detection limit of only 6.25 IU/mL and 1.48 IU/mL respectively.

Conclusion: Serological and molecular assays are predominant and reliable methods for HBV detection. Automated systems are highly sensitive and quantify HBV DNA and serological markers for monitoring.

Citing Articles

Detection technology and clinical applications of serum viral products of hepatitis B virus infection.

Liu Y, Wu D, Zhang K, Ren R, Liu Y, Zhang S Front Cell Infect Microbiol. 2024; 14:1402001.

PMID: 39035352 PMC: 11257880. DOI: 10.3389/fcimb.2024.1402001.

cccDNA-Targeted Drug Screen Reveals a Class of Antihistamines as Suppressors of HBV Genome Levels.

Ren E, Zhuo N, Goh Z, Bonne I, Malleret B, Ko H Biomolecules. 2023; 13(10).

PMID: 37892121 PMC: 10604930. DOI: 10.3390/biom13101438.

Comparative prevalence of hepatitis B virus infection among pregnant women accessing free maternal care in a tertiary hospital in Ghana.

Antuamwine B, Herchel E, Mishio Bawa E PLoS One. 2022; 17(3):e0263651.

PMID: 35245287 PMC: 8896678. DOI: 10.1371/journal.pone.0263651.

Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application.

Chen X, Wang S, Tan Y, Huang J, Yang X, Li S Front Bioeng Biotechnol. 2021; 9:731415.

PMID: 34595159 PMC: 8477041. DOI: 10.3389/fbioe.2021.731415.

Multiple Cross Displacement Amplification Linked with Nanoparticles-Based Lateral Flow Biosensor in Screening of Hepatitis B Virus in Clinical Application.

Chen X, Zhou Q, Dong S, Wang S, Liu R, Wu X Infect Drug Resist. 2021; 14:1219-1229.

PMID: 33790592 PMC: 8007573. DOI: 10.2147/IDR.S297645.

References

Magnius L, Espmark A . A new antigen complex co-occurring with Australia antigen. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972; 80(2):335-7. DOI: 10.1111/j.1699-0463.1972.tb00167.x. View

Mendy M, Kirk G, van der Sande M, Jeng-Barry A, Lesi O, Hainaut P . Hepatitis B surface antigenaemia and alpha-foetoprotein detection from dried blood spots: applications to field-based studies and to clinical care in hepatitis B virus endemic areas. J Viral Hepat. 2005; 12(6):642-7. DOI: 10.1111/j.1365-2893.2005.00641.x. View

Sun S, Meng S, Zhang R, Zhang K, Wang L, Li J . Development of a new duplex real-time polymerase chain reaction assay for hepatitis B viral DNA detection. Virol J. 2011; 8:227. PMC: 3116493. DOI: 10.1186/1743-422X-8-227. View

Yao C, Zhu T, Tang J, Wu R, Chen Q, Chen M . Hybridization assay of hepatitis B virus by QCM peptide nucleic acid biosensor. Biosens Bioelectron. 2007; 23(6):879-85. DOI: 10.1016/j.bios.2007.09.003. View

ROBINSON W, Clayton D, Greenman R . DNA of a human hepatitis B virus candidate. J Virol. 1974; 14(2):384-91. PMC: 355524. DOI: 10.1128/JVI.14.2.384-391.1974. View

Gupta E, Pandey P, Kumar A, Sharma M, Sarin S . Correlation between two chemiluminescence based assays for quantification of hepatitis B surface antigen in patients with chronic hepatitis B infection. Indian J Med Microbiol. 2015; 33(1):96-100. DOI: 10.4103/0255-0857.148400. View

Satoh K, Iwata-Takakura A, Yoshikawa A, Gotanda Y, Tanaka T, Yamaguchi T . A new method of concentrating hepatitis B virus (HBV) DNA and HBV surface antigen: an application of the method to the detection of occult HBV infection. Vox Sang. 2009; 95(3):174-80. DOI: 10.1111/j.1423-0410.2008.01091.x. View

Huang J, Liu Y, Wang J, Xu Z, Yang Y, Shen F . Next generation digital PCR measurement of hepatitis B virus copy number in formalin-fixed paraffin-embedded hepatocellular carcinoma tissue. Clin Chem. 2014; 61(1):290-6. DOI: 10.1373/clinchem.2014.230227. View

Khadem-Ansari M, Omrani M, Rasmi Y, Ghavam A . Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory. Adv Biomed Res. 2014; 3:116. PMC: 4063113. DOI: 10.4103/2277-9175.133178. View

10.

Cho Y, Kim J, Lee Y, Kim Y, Namkoong K, Lim H . Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus. Biosens Bioelectron. 2005; 21(11):2161-9. DOI: 10.1016/j.bios.2005.10.005. View

11.

Park Y, Kim B, Choi K, Shin D, Lee M, Cho Y . A novel multiplex real-time PCR assay for the concurrent detection of hepatitis A, B and C viruses in patients with acute hepatitis. PLoS One. 2012; 7(11):e49106. PMC: 3493491. DOI: 10.1371/journal.pone.0049106. View

12.

Weber B, Van der Taelem-Brule N, Berger A, Simon F, Geudin M, Ritter J . Evaluation of a new automated assay for hepatitis B surface antigen (HBsAg) detection VIDAS HBsAg Ultra. J Virol Methods. 2006; 135(1):109-17. DOI: 10.1016/j.jviromet.2006.02.009. View

13.

Yeh M, Huang C, Hsieh M, Huang J, Dai C, Yu M . Comparison of the Abbott RealTime HBV assay with the Roche Cobas AmpliPrep/Cobas TaqMan HBV assay for HBV DNA detection and quantification. J Clin Virol. 2014; 60(3):206-14. DOI: 10.1016/j.jcv.2014.04.008. View

14.

Chevaliez S, Bouvier-Alias M, Laperche S, Hezode C, Pawlotsky J . Performance of version 2.0 of the Cobas AmpliPrep/Cobas TaqMan real-time PCR assay for hepatitis B virus DNA quantification. J Clin Microbiol. 2010; 48(10):3641-7. PMC: 2953086. DOI: 10.1128/JCM.01306-10. View

15.

Gerlich W . Medical virology of hepatitis B: how it began and where we are now. Virol J. 2013; 10:239. PMC: 3729363. DOI: 10.1186/1743-422X-10-239. View

16.

Prince A . An antigen detected in the blood during the incubation period of serum hepatitis. Proc Natl Acad Sci U S A. 1968; 60(3):814-21. PMC: 225124. DOI: 10.1073/pnas.60.3.814. View

17.

Li Z, Hong G, Hu B, Liang M, Xu J, Li L . Suitability of yeast- and Escherichia coli-expressed hepatitis B virus core antigen derivatives for detection of anti-HBc antibodies in human sera. Protein Expr Purif. 2007; 56(2):293-300. DOI: 10.1016/j.pep.2007.08.004. View

18.

Maity S, Nandi S, Biswas S, Sadhukhan S, Saha M . Performance and diagnostic usefulness of commercially available enzyme linked immunosorbent assay and rapid kits for detection of HIV, HBV and HCV in India. Virol J. 2012; 9:290. PMC: 3568713. DOI: 10.1186/1743-422X-9-290. View

19.

Chen L, Liu F, Fan X, Gao J, Chen N, Wong T . Detection of hepatitis B surface antigen, hepatitis B core antigen, and hepatitis B virus DNA in parotid tissues. Int J Infect Dis. 2008; 13(1):20-3. DOI: 10.1016/j.ijid.2008.03.015. View

20.

Trepo C . A brief history of hepatitis milestones. Liver Int. 2013; 34 Suppl 1:29-37. DOI: 10.1111/liv.12409. View