Stage-specific Assembly Events of the 6-MDa Small-subunit Processome Initiate Eukaryotic Ribosome Biogenesis

Overview

Journal Nat Struct Mol Biol

Specialties Cell Biology
Molecular Biology

Date 2015 Oct 20

PMID 26479197

Citations 76

Authors

Malik Chaker-Margot

Mirjam Hunziker

Jonas Barandun

Brian D Dill

Sebastian Klinge

Affiliations

Soon will be listed here.

Abstract

Eukaryotic ribosome biogenesis involves a plethora of ribosome-assembly factors, and their temporal order of association with preribosomal RNA is largely unknown. By using Saccharomyces cerevisiae as a model organism, we developed a system that recapitulates and arrests ribosome assembly at early stages, thus providing in vivo snapshots of nascent preribosomal particles. Here we report the stage-specific order in which 70 ribosome-assembly factors associate with preribosomal RNA domains, thereby forming the 6-MDa small-subunit processome.

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