Single Nucleotide Polymorphism Genotyping and Distribution of Coxiella Burnetii Strains from Field Samples in Belgium

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2015 Oct 18

PMID 26475104

Citations 4

Authors

Fabiana Dal Pozzo

Benedicte Renaville

Ludovic Martinelle

Robert Renaville

Christine Thys

Francois Smeets

Nathalie Kirschvink

Fabien Gregoire

Laurent Delooz

Guy Czaplicki

Claude Saegerman

Affiliations

Soon will be listed here.

Abstract

The genotypic characterization of Coxiella burnetii provides useful information about the strains circulating at the farm, region, or country level and may be used to identify the source of infection for animals and humans. The aim of the present study was to investigate the strains of C. burnetii circulating in caprine and bovine Belgian farms using a single nucleotide polymorphism (SNP) technique. Direct genotyping was applied to different samples (bulk tank milk, individual milk, vaginal swab, fetal product, and air sample). Besides the well-known SNP genotypes, unreported ones were found in bovine and caprine samples, increasing the variability of the strains found in the two species in Belgium. Moreover, multiple genotypes were detected contemporarily in caprine farms at different years of sampling and by using different samples. Interestingly, certain SNP genotypes were detected in both bovine and caprine samples, raising the question of interspecies transmission of the pathogen.

Citing Articles

Phylogeography of Human and Animal Strains: Genetic Fingerprinting of Q Fever in Belgium.

Tomaiuolo S, Boarbi S, Fancello T, Michel P, Desqueper D, Gregoire F Front Cell Infect Microbiol. 2021; 10:625576.

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Monitoring Infection in Naturally Infected Dairy Sheep Flocks Throughout Four Lambing Seasons and Investigation of Viable Bacteria.

Alvarez-Alonso R, Zendoia I, Barandika J, Jado I, Hurtado A, Lopez C Front Vet Sci. 2020; 7:352.

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Stable levels of Coxiella burnetii prevalence in dairy sheep flocks but changes in genotype distribution after a 10-year period in northern Spain.

Alvarez-Alonso R, Barandika J, Ruiz-Fons F, Ortega-Araiztegi I, Jado I, Hurtado A Acta Vet Scand. 2018; 60(1):75.

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From Q Fever to Coxiella burnetii Infection: a Paradigm Change.

Eldin C, Melenotte C, Mediannikov O, Ghigo E, Million M, Edouard S Clin Microbiol Rev. 2016; 30(1):115-190.

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