The Autophagy GABARAPL1 Gene is Epigenetically Regulated in Breast Cancer Models

Overview

Journal BMC Cancer

Publisher Biomed Central

Specialty Oncology

Date 2015 Oct 18

PMID 26474850

Citations 29

Authors

Eric Hervouet

Aurore Claude-Taupin

Thierry Gauthier

Valerie Perez

Annick Fraichard

Pascale Adami

Gilles Despouy

Franck Monnien

Marie-Paule Algros

Michele Jouvenot

Regis Delage-Mourroux

Michael Boyer-Guittaut

Affiliations

Soon will be listed here.

Abstract

Background: The GABARAP family members (GABARAP, GABARAPL1/GEC1 and GABARAPL2 /GATE-16) are involved in the intracellular transport of receptors and the autophagy pathway. We previously reported that GABARAPL1 expression was frequently downregulated in cancer cells while a high GABARAPL1 expression is a good prognosis marker for patients with lymph node-positive breast cancer.

Methods: In this study, we asked using qRT-PCR, western blotting and epigenetic quantification whether the expression of the GABARAP family was regulated in breast cancer by epigenetic modifications.

Results: Our data demonstrated that a specific decrease of GABARAPL1 expression in breast cancers was associated with both DNA methylation and histone deacetylation and that CREB-1 recruitment on GABARAPL1 promoter was required for GABARAPL1 expression.

Conclusions: Our work strongly suggests that epigenetic inhibitors and CREB-1 modulators may be used in the future to regulate autophagy in breast cancer cells.

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