Lysosome-associated Membrane Glycoprotein (LAMP)--preliminary Study on a Hidden Antigen Target for Vaccination Against Schistosomiasis

Overview

Journal Sci Rep

Specialty Science

Date 2015 Oct 17

PMID 26472258

Citations 7

Authors

Sujeevi S K Nawaratna

Geoffrey N Gobert

Charlene Willis

Jason Mulvenna

Andreas Hofmann

Donald P McManus

Malcolm K Jones

Affiliations

Soon will be listed here.

Abstract

Our previously reported gene atlasing of schistosome tissues revealed transcripts that were highly enriched in the digestive tract of Schistosoma mansoni. From these, we selected two candidates, Sm-LAMP and Sm-NPC2 for testing as vaccine targets. The two molecules were selected on the basis of relatively high expression in the gastrodermis, their potentially important biological function, divergence from homologous molecules of the host and possible apical membrane expression in the gastrodermis. Bacterially expressed recombinant peptides corresponding to regions excluding trans-membrane domains of the selected vaccine targets were used in blinded vaccine trials in CBA mice using alum-CpG as adjuvant. Vaccine trials using the recombinant insoluble Sm-LAMP protein showed 16-25% significant reduction in total worm burden. Faecal egg count reduction was 52% and 60% in two trials, respectively, with similar results for the solubly expressed protein. Liver egg burden was reduced significantly (20% and 38%) with an insoluble recombinant Sm-LAMP in two trials, but not with the soluble recombinant form. Parasite fecundity was not affected by either Sm-LAMP protein preparations in the trials. It is concluded that Sm-LAMP may provide limited protection towards S. mansoni infections but could be used in combination with other vaccine candidates, to provide more comprehensive protection.

Citing Articles

HGPRT and PNP: Recombinant Enzymes from and Their Role in Immunotherapy during Experimental Murine Schistosomiasis.

de Lima Fragelli B, Fattori A, de Almeida Montija E, de Almeida Rodolpho J, de Castro C, de Godoy K Pathogens. 2023; 12(4).

PMID: 37111413 PMC: 10144537. DOI: 10.3390/pathogens12040527.

Effects of Immunization with Recombinant Enzymes AK and HGPRT: Murine Infection Control.

Fattori A, Montija E, Fragelli B, Correia R, de Castro C, Romanello L Pathogens. 2023; 12(1).

PMID: 36678417 PMC: 9866087. DOI: 10.3390/pathogens12010069.

Adult schistosomes have an epithelial bacterial population distinct from the surrounding mammalian host blood.

Gobert G, McManus D, McMullan G, Creevey C, Carson J, Jones M PLoS One. 2022; 17(1):e0263188.

PMID: 35085360 PMC: 8794206. DOI: 10.1371/journal.pone.0263188.

The Search for a Schistosomiasis Vaccine: Australia's Contribution.

McManus D Vaccines (Basel). 2021; 9(8).

PMID: 34451997 PMC: 8402410. DOI: 10.3390/vaccines9080872.

A comprehensive and critical overview of schistosomiasis vaccine candidates.

Al-Naseri A, Al-Absi S, El Ridi R, Mahana N J Parasit Dis. 2021; 45(2):557-580.

PMID: 33935395 PMC: 8068781. DOI: 10.1007/s12639-021-01387-w.

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