Down-regulation of MiR-129-5p Via the Twist1-Snail Feedback Loop Stimulates the Epithelial-mesenchymal Transition and is Associated with Poor Prognosis in Breast Cancer

Overview

Journal Oncotarget

Specialty Oncology

Date 2015 Oct 14

PMID 26460733

Citations 38

Authors

Yue Yu

Ying Zhao

Xiao-Hu Sun

Jie Ge

Bin Zhang

Xin Wang

Xu-Chen Cao

Affiliations

Soon will be listed here.

Abstract

The epithelial to mesenchymal transition (EMT) plays a pivotal role in breast cancer progression. We found that overexpression of miR-129-5p reversed EMT, whereas depletion of miR-129-5p induced EMT in breast cancer cells. We demonstrated that Twist1 is a direct target of miR-129-5p. Both Twist1 and Snail transcriptionally suppressed miR-129-5p expression. Levels of miR-129-5p were low in breast cancer tissues. miR-129-5p down-regulation correlated with advanced clinical stage and poor prognosis in patients with breast cancer. miR-129-5p expression negatively correlated with Twist1 and Snail expression. Thus, miR-129-5p down-regulation fosters EMT in breast cancer by increasing Twist1-Snail and activating a negative feedback loop.

Citing Articles

Pivotal Role of miRNA-lncRNA Interactions in Human Diseases.

Pooresmaeil F, Azadi S, Hasannejad-Asl B, Takamoli S, Bolhassani A Mol Biotechnol. 2024; .

PMID: 39673006 DOI: 10.1007/s12033-024-01343-y.

Unique miRomics Expression Profiles in -Infected Mandibles during Periodontitis Using Machine Learning.

Aravindraja C, Jeepipalli S, Duncan W, Vekariya K, Bahadekar S, Chan E Int J Mol Sci. 2023; 24(22).

PMID: 38003583 PMC: 10671577. DOI: 10.3390/ijms242216393.

The Expression Levels of Circulating miR-129 and miR-203a in Association with Breast Cancer and Related Metastasis.

Ghalkhani E, Akbari M, Izadi P, Mahmoodzadeh H, Kamali F Iran J Public Health. 2023; 51(12):2808-2816.

PMID: 36742232 PMC: 9874213. DOI: 10.18502/ijph.v51i12.11472.

Review article epithelial to mesenchymal transition‑associated microRNAs in breast cancer.

Javdani H, Mollaei H, Karimi F, Mahmoudi S, Farahi A, Mirzaei-Parsa M Mol Biol Rep. 2022; 49(10):9963-9973.

PMID: 35716288 DOI: 10.1007/s11033-022-07553-4.

MiRNA-mediated EMT and CSCs in cancer chemoresistance.

Dong B, Li S, Zhu S, Yi M, Luo S, Wu K Exp Hematol Oncol. 2021; 10(1):12.

PMID: 33579377 PMC: 7881653. DOI: 10.1186/s40164-021-00206-5.

References

Yu J, Xie F, Bao X, Chen W, Xu Q . miR-300 inhibits epithelial to mesenchymal transition and metastasis by targeting Twist in human epithelial cancer. Mol Cancer. 2014; 13:121. PMC: 4040483. DOI: 10.1186/1476-4598-13-121. View

Chen X, Zhang L, Zhang T, Hao M, Zhang X, Zhang J . Methylation-mediated repression of microRNA 129-2 enhances oncogenic SOX4 expression in HCC. Liver Int. 2013; 33(3):476-86. DOI: 10.1111/liv.12097. View

Wu Q, Yang Z, Xia L, Nie Y, Wu K, Shi Y . Methylation of miR-129-5p CpG island modulates multi-drug resistance in gastric cancer by targeting ABC transporters. Oncotarget. 2014; 5(22):11552-63. PMC: 4294356. DOI: 10.18632/oncotarget.2594. View

Yang Y, Huang J, Zhang X, Shen L . MiR-129-2 functions as a tumor suppressor in glioma cells by targeting HMGB1 and is down-regulated by DNA methylation. Mol Cell Biochem. 2015; 404(1-2):229-39. PMC: 4544670. DOI: 10.1007/s11010-015-2382-6. View

Ma N, Chen F, Shen S, Chen W, Chen L, Su Q . MicroRNA-129-5p inhibits hepatocellular carcinoma cell metastasis and invasion via targeting ETS1. Biochem Biophys Res Commun. 2015; 461(4):618-23. DOI: 10.1016/j.bbrc.2015.04.075. View

Long X, Zhou Y, Peng A, Zhang Z, Chen X, Chen W . Demethylation-mediated miR-129-5p up-regulation inhibits malignant phenotype of osteogenic osteosarcoma by targeting Homo sapiens valosin-containing protein (VCP). Tumour Biol. 2015; 36(5):3799-806. DOI: 10.1007/s13277-014-3021-7. View

Li J, Wang H, Ke H, Ni S . MiR-129 regulates MMP9 to control metastasis of non-small cell lung cancer. Tumour Biol. 2015; 36(8):5785-90. DOI: 10.1007/s13277-015-3247-z. View

Yang J, Mani S, Liu Donaher J, Ramaswamy S, Itzykson R, Come C . Twist, a master regulator of morphogenesis, plays an essential role in tumor metastasis. Cell. 2004; 117(7):927-39. DOI: 10.1016/j.cell.2004.06.006. View

Maeda M, Johnson K, Wheelock M . Cadherin switching: essential for behavioral but not morphological changes during an epithelium-to-mesenchyme transition. J Cell Sci. 2005; 118(Pt 5):873-87. DOI: 10.1242/jcs.01634. View

10.

Tili E, Michaille J, Croce C . MicroRNAs play a central role in molecular dysfunctions linking inflammation with cancer. Immunol Rev. 2013; 253(1):167-84. DOI: 10.1111/imr.12050. View

11.

Kang M, Li Y, Liu W, Wang R, Tang A, Hao H . miR-129-2 suppresses proliferation and migration of esophageal carcinoma cells through downregulation of SOX4 expression. Int J Mol Med. 2013; 32(1):51-8. DOI: 10.3892/ijmm.2013.1384. View

12.

Karaayvaz M, Zhai H, Ju J . miR-129 promotes apoptosis and enhances chemosensitivity to 5-fluorouracil in colorectal cancer. Cell Death Dis. 2013; 4:e659. PMC: 3702282. DOI: 10.1038/cddis.2013.193. View

13.

Wang R, Ma J, Wu Q, Xia J, Miele L, Sarkar F . Functional role of miR-34 family in human cancer. Curr Drug Targets. 2013; 14(10):1185-91. DOI: 10.2174/13894501113149990191. View

14.

Yu X, Song H, Xia T, Han S, Xiao B, Luo L . Growth inhibitory effects of three miR-129 family members on gastric cancer. Gene. 2013; 532(1):87-93. DOI: 10.1016/j.gene.2013.09.048. View

15.

Li M, Tian L, Wang L, Yao H, Zhang J, Lu J . Down-regulation of miR-129-5p inhibits growth and induces apoptosis in laryngeal squamous cell carcinoma by targeting APC. PLoS One. 2013; 8(10):e77829. PMC: 3806837. DOI: 10.1371/journal.pone.0077829. View

16.

DAmato N, Howe E, Richer J . MicroRNA regulation of epithelial plasticity in cancer. Cancer Lett. 2012; 341(1):46-55. DOI: 10.1016/j.canlet.2012.11.054. View

17.

Parker J, Mullins M, Cheang M, Leung S, Voduc D, Vickery T . Supervised risk predictor of breast cancer based on intrinsic subtypes. J Clin Oncol. 2009; 27(8):1160-7. PMC: 2667820. DOI: 10.1200/JCO.2008.18.1370. View

18.

Dyrskjot L, Ostenfeld M, Bramsen J, Silahtaroglu A, Lamy P, Ramanathan R . Genomic profiling of microRNAs in bladder cancer: miR-129 is associated with poor outcome and promotes cell death in vitro. Cancer Res. 2009; 69(11):4851-60. DOI: 10.1158/0008-5472.CAN-08-4043. View

19.

Kalluri R, Weinberg R . The basics of epithelial-mesenchymal transition. J Clin Invest. 2009; 119(6):1420-8. PMC: 2689101. DOI: 10.1172/JCI39104. View

20.

Ogawa R, Ishiguro H, Kuwabara Y, Kimura M, Mitsui A, Katada T . Expression profiling of micro-RNAs in human esophageal squamous cell carcinoma using RT-PCR. Med Mol Morphol. 2009; 42(2):102-9. DOI: 10.1007/s00795-009-0443-1. View