Regulation of HepG2 Cell Apoptosis by Hepatitis C Virus (HCV) Core Protein Via the Sirt1-p53-bax Pathway

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2015 Oct 14

PMID 26459383

Citations 10

Authors

Shenghu Feng

Min Li

Jinqian Zhang

Shunai Liu

Qi Wang

Min Quan

Mengran Zhang

Jun Cheng

Affiliations

Soon will be listed here.

Abstract

Hepatitis C virus (HCV) core protein stimulates many signaling pathways related to apoptosis inhibition resulting in hepatocellular carcinoma (HCC). It has been reported that sirt1 is involved in regulating apoptosis; therefore, we investigated the influence of HCV core protein on sirt1 expression and apoptosis in human HepG2 cells. Our study showed that HCV core protein inhibited apoptosis of HepG2 cells as well as caspase-3 expression and activity (P < 0.05). At the same time, sirt1 expression was increased at both the mRNA (P < 0.05) and protein (P < 0.05) levels. Furthermore, apoptosis inhibition was reversed when sirt1 was knocked down (P < 0.05). Our study provides further evidence that the sirt1-p53-Bax signaling pathway plays an important role in regulating the suppression of cell apoptosis induced by HCV core protein.

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