Genetic Variation in MiR-146a Is Not Associated with Susceptibility to IgA Nephropathy in Adults from a Chinese Han Population

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Oct 2

PMID 26426696

Citations 5

Authors

Bin Yang

Wei Wei

Yunying Shi

Zhuochun Huang

Bei Cai

Junlong Zhang

Binwu Ying

Lanlan Wang

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNA 146a (miR-146a) is a 19 to 23 nucleotide long, small non-coding RNA with gene regulatory functions that has influence on the pathogenesis of many diseases. A single nucleotide polymorphism (rs2910164 C>G) in pre-miR-146a is correlated with the expression of miR-146a. The aim of this study was to perform an association analysis of rs2910164 with IgA nephropathy in adult patients from a Chinese Han population.

Methods: A total of 145 patients with renal biopsy-proved IgA nephropathy (IgAN) and 179 healthy controls were recruited to the current study. rs2910164 was genotyped by the polymerase chain reaction (PCR) and high-resolution melting methods (HRM). Clinical characteristics and pathology grading of patients with IgAN were recorded at the time of kidney biopsy.

Result: There were significant differences among the population of patients grouped by different age of onset in a co-dominant model (CG vs. CC vs. GG) (p = 0.033) and a recessive model (CG+CC vs. GG) (p = 0.001). However, no significant difference was observed in the distribution of genotypes between cases and controls (p = 0.144). There was also no significant difference between rs2910164 and patient quantitative traits (all p > 0.003) or different pathology grading (Lee's grading system and tubular atrophy/interstitial fibrosis in the Oxford classification) (all p > 0.05).

Conclusions: There was no association of rs2910164 with susceptibility to IgAN in adults from a Chinese Han population. However, rs2910164 was correlated with the age of onset of IgAN in adult patients.

Citing Articles

Analysis of IL-17A, IL-17F, and miR-146a-5p Prior to Transplantation and Their Role in Kidney Transplant Recipients.

Wysoczanska B, Dratwa M, Nieszporek A, Niepieklo-Miniewska W, Kaminska D, Ramus T J Clin Med. 2024; 13(10).

PMID: 38792460 PMC: 11122464. DOI: 10.3390/jcm13102920.

Meta-Analysis of miRNA Variants Associated with Susceptibility to Autoimmune Disease.

Zhang J, Tan H, Cao Q, Su G, Yang P Dis Markers. 2021; 2021:9978460.

PMID: 34659590 PMC: 8519726. DOI: 10.1155/2021/9978460.

MicroRNAs in IgA nephropathy.

Yao X, Zhai Y, An H, Gao J, Chen Y, Zhang W Ren Fail. 2021; 43(1):1298-1310.

PMID: 34547971 PMC: 8462925. DOI: 10.1080/0886022X.2021.1977320.

The Non-Coding RNA Landscape in IgA Nephropathy-Where Are We in 2021?.

Pawluczyk I, Selvaskandan H, Barratt J J Clin Med. 2021; 10(11).

PMID: 34071162 PMC: 8198207. DOI: 10.3390/jcm10112369.

MicroRNAs: a new avenue to understand, investigate and treat immunoglobulin A nephropathy?.

Selvaskandan H, Pawluczyk I, Barratt J Clin Kidney J. 2018; 11(1):29-37.

PMID: 29423198 PMC: 5798124. DOI: 10.1093/ckj/sfx096.

References

Yu H, Chiang B . Diagnosis and classification of IgA nephropathy. Autoimmun Rev. 2014; 13(4-5):556-9. DOI: 10.1016/j.autrev.2014.01.030. View

Guo H, Ingolia N, Weissman J, Bartel D . Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature. 2010; 466(7308):835-40. PMC: 2990499. DOI: 10.1038/nature09267. View

Lofgren S, Frostegard J, Truedsson L, Pons-Estel B, DAlfonso S, Witte T . Genetic association of miRNA-146a with systemic lupus erythematosus in Europeans through decreased expression of the gene. Genes Immun. 2012; 13(3):268-74. PMC: 3640319. DOI: 10.1038/gene.2011.84. View

Hou J, Wang P, Lin L, Liu X, Ma F, An H . MicroRNA-146a feedback inhibits RIG-I-dependent Type I IFN production in macrophages by targeting TRAF6, IRAK1, and IRAK2. J Immunol. 2009; 183(3):2150-8. DOI: 10.4049/jimmunol.0900707. View

ONeill L, Sheedy F, McCoy C . MicroRNAs: the fine-tuners of Toll-like receptor signalling. Nat Rev Immunol. 2011; 11(3):163-75. DOI: 10.1038/nri2957. View

Floege J, Moura I, Daha M . New insights into the pathogenesis of IgA nephropathy. Semin Immunopathol. 2014; 36(4):431-42. DOI: 10.1007/s00281-013-0411-7. View

Singh S, Rai G, Aggarwal A . Association of microRNA-146a and its target gene IRAK1 polymorphism with enthesitis related arthritis category of juvenile idiopathic arthritis. Rheumatol Int. 2014; 34(10):1395-400. DOI: 10.1007/s00296-014-3001-7. View

Lee H, Lee M, Lee S, Lee S, Lee E, Lee E . Histological grading of IgA nephropathy predicting renal outcome: revisiting H. S. Lee's glomerular grading system. Nephrol Dial Transplant. 2004; 20(2):342-8. DOI: 10.1093/ndt/gfh633. View

Geddes C, Rauta V, Gronhagen-Riska C, Bartosik L, Jardine A, Ibels L . A tricontinental view of IgA nephropathy. Nephrol Dial Transplant. 2003; 18(8):1541-8. DOI: 10.1093/ndt/gfg207. View

10.

Loktionov A . Common gene polymorphisms, cancer progression and prognosis. Cancer Lett. 2004; 208(1):1-33. DOI: 10.1016/j.canlet.2004.02.009. View

11.

Shao Y, Li J, Cai Y, Xie Y, Ma G, Li Y . The functional polymorphisms of miR-146a are associated with susceptibility to severe sepsis in the Chinese population. Mediators Inflamm. 2014; 2014:916202. PMC: 3950573. DOI: 10.1155/2014/916202. View

12.

Gauderman W . Candidate gene association analysis for a quantitative trait, using parent-offspring trios. Genet Epidemiol. 2003; 25(4):327-38. DOI: 10.1002/gepi.10262. View

13.

Gauderman W . Sample size requirements for association studies of gene-gene interaction. Am J Epidemiol. 2002; 155(5):478-84. DOI: 10.1093/aje/155.5.478. View

14.

Wyatt R, Julian B . IgA nephropathy. N Engl J Med. 2013; 368(25):2402-14. DOI: 10.1056/NEJMra1206793. View

15.

Gauderman W . Sample size requirements for matched case-control studies of gene-environment interaction. Stat Med. 2002; 21(1):35-50. DOI: 10.1002/sim.973. View

16.

Wang G, Kwan B, Lai F, Chow K, Kam-Tao Li P, Szeto C . Expression of microRNAs in the urinary sediment of patients with IgA nephropathy. Dis Markers. 2010; 28(2):79-86. PMC: 3833417. DOI: 10.3233/DMA-2010-0687. View

17.

Olivieri F, Procopio A, Montgomery R . Effect of aging on microRNAs and regulation of pathogen recognition receptors. Curr Opin Immunol. 2014; 29:29-37. PMC: 4119513. DOI: 10.1016/j.coi.2014.03.006. View

18.

Yang B, Zhang J, Shi Y, Li D, Chen J, Huang Z . Association study of single nucleotide polymorphisms in pre-miRNA and rheumatoid arthritis in a Han Chinese population. Mol Biol Rep. 2010; 38(8):4913-9. DOI: 10.1007/s11033-010-0633-x. View

19.

Li K, Du Y, Jiang B, He J . Increased microRNA-155 and decreased microRNA-146a may promote ocular inflammation and proliferation in Graves' ophthalmopathy. Med Sci Monit. 2014; 20:639-43. PMC: 3999163. DOI: 10.12659/MSM.890686. View

20.

Liu L, Gu H, Liu H, Jiao Y, Li K, Zhao Y . Protective effect of resveratrol against IL-1β-induced inflammatory response on human osteoarthritic chondrocytes partly via the TLR4/MyD88/NF-κB signaling pathway: an "in vitro study". Int J Mol Sci. 2014; 15(4):6925-40. PMC: 4013670. DOI: 10.3390/ijms15046925. View