Mesoporous CLEAs-silica Composite Microparticles with High Activity and Enhanced Stability

Overview

Journal Sci Rep

Specialty Science

Date 2015 Sep 17

PMID 26374188

Citations 3

Authors

Jiandong Cui

Shiru Jia

Longhao Liang

Yamin Zhao

Yuxiao Feng

Affiliations

Soon will be listed here.

Abstract

A novel enzyme immobilization approach was used to generate mesoporous enzymes-silica composite microparticles by co-entrapping gelatinized starch and cross-linked phenylalanine ammonia lyase (PAL) aggregates (CLEAs) containing gelatinized starch into biomemitic silica and subsequently removing the starch by α-amylase treatment. During the preparation process, the gelatinzed starch served as a pore-forming agent to create pores in CLEAs and biomimetic silica. The resulting mesoporous CLEAs-silica composite microparticles exhibited higher activity and stability than native PAL, conventional CLEAs, and PAL encapsulated in biomimetic silica. Furthermore, the mesoporous CLEAs-silica composite microparticles displayed good reusability due to its suitable size and mechanical properties, and had excellent stability for storage. The superior catalytic performances were attributed to the combinational unique structure from the intra-cross-linking among enzyme aggregates and hard mesoporous silica shell, which not only decreased the enzyme-support negative interaction and mass-transfer limitations, but also improved the mechanical properties and monodispersity. This approach will be highly beneficial for preparing various bioactive mesoporous composites with excellent catalytic performance.

Citing Articles

Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme.

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