Bu-Shen-Ning-Xin Decoction Ameliorated the Osteoporotic Phenotype of Ovariectomized Mice Without Affecting the Serum Estrogen Concentration or Uterus

Overview

Journal Drug Des Devel Ther

Specialty Pharmacology

Date 2015 Sep 11

PMID 26357466

Citations 3

Authors

Ling Wang

Xue-Min Qiu

Yu-Yan Gui

Ying-Ping Xu

Hans-Jurgen Gober

Da-Jin Li

Affiliations

Soon will be listed here.

Abstract

Introduction: Bu-Shen-Ning-Xin Decoction (BSNXD), a traditional Chinese medicinal composition, has been used as a remedy for postmenopausal osteoporosis, but its effects on bone metabolism and the uterus have not been reported.

Purpose: We aimed to determine the respective effects of BSNXD on the bones and the uterus of ovariectomized (OVX) mice to evaluate the efficacy and safety of this herbal formula.

Materials And Methods: Postmenopausal osteoporosis animal models that were generated by ovariectomy were treated with BSNXD. Dual-energy X-ray absorptiometry was performed to analyze the bone mineral density, and histomorphometric analysis was performed to measure the parameters related to bone metabolism. Calcein labeling was performed to detect bone formation. The uteruses from the mice were weighed, and the histomorphometry was analyzed. Drug-derived serum was prepared to assess the 17-β-estradiol concentration via enzyme immunoassay.

Results: BSNXD administration ameliorated the osteoporotic phenotype of OVX mice, as evidenced by an increase in the bone mineral density and bone volume; these effects could not be abolished by the administration of the aromatase inhibitor letrozole. Moreover, BSNXD had no effect on the serum estrogen concentration or uterus.

Conclusion: These results suggest that BSNXD has ameliorating effects on bone loss due to estrogen deprivation without affecting the peripheral blood estrogen concentration or the uterus in OVX mice.

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References

Li F, Chow S, Cheung W, Chan F, Chen S, Leung L . The citrus flavonone hesperetin prevents letrozole-induced bone loss in a mouse model of breast cancer. J Nutr Biochem. 2012; 24(6):1112-6. DOI: 10.1016/j.jnutbio.2012.08.010. View

Huebner A, Schinke T, Priemel M, Schilling S, Schilling A, Emeson R . Calcitonin deficiency in mice progressively results in high bone turnover. J Bone Miner Res. 2006; 21(12):1924-34. DOI: 10.1359/jbmr.060820. View

Nawata H, Tanaka S, Takayanagi R, Sakai Y, Yanase T, Ikuyama S . Aromatase in bone cell: association with osteoporosis in postmenopausal women. J Steroid Biochem Mol Biol. 1995; 53(1-6):165-74. DOI: 10.1016/0960-0760(95)00031-t. View

Wang L, Qiu X, Hao Q, Li D . Anti-inflammatory effects of a Chinese herbal medicine in atherosclerosis via estrogen receptor β mediating nitric oxide production and NF-κB suppression in endothelial cells. Cell Death Dis. 2013; 4:e551. PMC: 3615733. DOI: 10.1038/cddis.2013.66. View

Body J . How to manage postmenopausal osteoporosis?. Acta Clin Belg. 2012; 66(6):443-7. DOI: 10.2143/ACB.66.6.2062612. View

Goto T, Hagiwara K, Shirai N, Yoshida K, Hagiwara H . Apigenin inhibits osteoblastogenesis and osteoclastogenesis and prevents bone loss in ovariectomized mice. Cytotechnology. 2014; 67(2):357-65. PMC: 4329293. DOI: 10.1007/s10616-014-9694-3. View

Cui J, Zhu M, Zhu S, Wang G, Xu Y, Geng D . Inhibitory effect of icariin on Ti-induced inflammatory osteoclastogenesis. J Surg Res. 2014; 192(2):447-53. DOI: 10.1016/j.jss.2014.05.038. View

Yang J, Cho S, An J, Jung J, Kim S, Kim S . Osteoblast-targeted overexpression of TAZ increases bone mass in vivo. PLoS One. 2013; 8(2):e56585. PMC: 3575506. DOI: 10.1371/journal.pone.0056585. View

Sah C, Aridogan I, Izol V, Erdogan S, Doran S . Effects of Long-term Administration of the Antiaging Hormone Dehydroepiandrosterone Sulfate on Rat Prostates and Testes as Androgen-Dependent Organs. Korean J Urol. 2013; 54(3):199-203. PMC: 3604575. DOI: 10.4111/kju.2013.54.3.199. View

10.

Magyar Z, Bekesi G, Racz K, Feher J, Schaff Z, Lengyel G . Increased total scavenger capacity and decreased liver fat content in rats fed dehydroepiandrosterone and its sulphate on a high-fat diet. Gerontology. 2010; 57(4):343-9. DOI: 10.1159/000321385. View

11.

Parfitt A, Drezner M, Glorieux F, Kanis J, MALLUCHE H, Meunier P . Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res. 1987; 2(6):595-610. DOI: 10.1002/jbmr.5650020617. View

12.

Song L, Zhao J, Zhang X, Li H, Zhou Y . Icariin induces osteoblast proliferation, differentiation and mineralization through estrogen receptor-mediated ERK and JNK signal activation. Eur J Pharmacol. 2013; 714(1-3):15-22. DOI: 10.1016/j.ejphar.2013.05.039. View

13.

Holmes C, Khan T, Owen C, Ciliberti N, Grynpas M, Stanford W . Longitudinal analysis of mesenchymal progenitors and bone quality in the stem cell antigen-1-null osteoporotic mouse. J Bone Miner Res. 2007; 22(9):1373-86. DOI: 10.1359/jbmr.070604. View

14.

Traish A, Kang H, Saad F, Guay A . Dehydroepiandrosterone (DHEA)--a precursor steroid or an active hormone in human physiology. J Sex Med. 2011; 8(11):2960-82. DOI: 10.1111/j.1743-6109.2011.02523.x. View

15.

Zhu S, Chen K, Lan Y, Zhang N, Jiang R, Hu J . Alendronate protects against articular cartilage erosion by inhibiting subchondral bone loss in ovariectomized rats. Bone. 2013; 53(2):340-9. DOI: 10.1016/j.bone.2012.12.044. View

16.

Wang L, Zhou G, Liu P, Song J, Liang Y, Yan X . Dissection of mechanisms of Chinese medicinal formula Realgar-Indigo naturalis as an effective treatment for promyelocytic leukemia. Proc Natl Acad Sci U S A. 2008; 105(12):4826-31. PMC: 2290784. DOI: 10.1073/pnas.0712365105. View

17.

Li J, Tawfeek H, Bedi B, Yang X, Adams J, Gao K . Ovariectomy disregulates osteoblast and osteoclast formation through the T-cell receptor CD40 ligand. Proc Natl Acad Sci U S A. 2010; 108(2):768-73. PMC: 3021053. DOI: 10.1073/pnas.1013492108. View

18.

Kohama S, Anderson C, Osterburg H, May P, Finch C . Oral administration of estradiol to young C57BL/6J mice induces age-like neuroendocrine dysfunctions in the regulation of estrous cycles. Biol Reprod. 1989; 41(2):227-32. DOI: 10.1095/biolreprod41.2.227. View

19.

Pinkerton J, Stovall D . Reproductive aging, menopause, and health outcomes. Ann N Y Acad Sci. 2010; 1204:169-78. DOI: 10.1111/j.1749-6632.2010.05526.x. View

20.

Rossouw J, Anderson G, Prentice R, LaCroix A, Kooperberg C, Stefanick M . Risks and benefits of estrogen plus progestin in healthy postmenopausal women: principal results From the Women's Health Initiative randomized controlled trial. JAMA. 2002; 288(3):321-33. DOI: 10.1001/jama.288.3.321. View