Berberine Regulated Lipid Metabolism in the Presence of C75, Compound C, and TOFA in Breast Cancer Cell Line MCF-7

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2015 Sep 10

PMID 26351511

Citations 10

Authors

Wen Tan

Zhangfeng Zhong

Shengpeng Wang

Zhanwei Suo

Xian Yang

Xiaodong Hu

Yitao Wang

Affiliations

Soon will be listed here.

Abstract

Berberine interfering with cancer reprogramming metabolism was confirmed in our previous study. Lipid metabolism and mitochondrial function were also the core parts in reprogramming metabolism. In the presence of some energy-related inhibitors, including C75, compound C, and TOFA, the discrete roles of berberine in lipid metabolism and mitochondrial function were elucidated. An altered lipid metabolism induced by berberine was observed under the inhibition of FASN, AMPK, and ACC in breast cancer cell MCF-7. And the reversion of berberine-induced lipid suppression indicated that ACC inhibition might be involved in that process instead of FASN inhibition. A robust apoptosis induced by berberine even under the inhibition of AMPK and lipid synthesis was also indicated. Finally, mitochondrial function regulation under the inhibition of AMPK and ACC might be in an ACL-independent manner. Undoubtedly, the detailed mechanisms of berberine interfering with lipid metabolism and mitochondrial function combined with energy-related inhibitors need further investigation, including the potential compensatory mechanisms for ATP production and the upregulation of ACL.

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References

Lin X, Wu G, Huo W, Zhang Y, Jin F . Resveratrol induces apoptosis associated with mitochondrial dysfunction in bladder carcinoma cells. Int J Urol. 2012; 19(8):757-64. DOI: 10.1111/j.1442-2042.2012.03024.x. View

Kim H, Kim M, Kim E, Yang Y, Lee M, Lim J . Berberine-induced AMPK activation inhibits the metastatic potential of melanoma cells via reduction of ERK activity and COX-2 protein expression. Biochem Pharmacol. 2011; 83(3):385-94. DOI: 10.1016/j.bcp.2011.11.008. View

Ballot C, Kluza J, Lancel S, Martoriati A, Hassoun S, Mortier L . Inhibition of mitochondrial respiration mediates apoptosis induced by the anti-tumoral alkaloid lamellarin D. Apoptosis. 2010; 15(7):769-81. DOI: 10.1007/s10495-010-0471-2. View

Gowans G, Hawley S, Ross F, Grahame Hardie D . AMP is a true physiological regulator of AMP-activated protein kinase by both allosteric activation and enhancing net phosphorylation. Cell Metab. 2013; 18(4):556-66. PMC: 3791399. DOI: 10.1016/j.cmet.2013.08.019. View

Chen X, Wei S, Ma Y, Lu J, Niu G, Xue Y . Quantitative proteomics analysis identifies mitochondria as therapeutic targets of multidrug-resistance in ovarian cancer. Theranostics. 2014; 4(12):1164-75. PMC: 4183995. DOI: 10.7150/thno.8502. View

Heerdt B, Houston M, Augenlicht L . Short-chain fatty acid-initiated cell cycle arrest and apoptosis of colonic epithelial cells is linked to mitochondrial function. Cell Growth Differ. 1997; 8(5):523-32. View

Martinez-Outschoorn U, Goldberg A, Lin Z, Ko Y, Flomenberg N, Wang C . Anti-estrogen resistance in breast cancer is induced by the tumor microenvironment and can be overcome by inhibiting mitochondrial function in epithelial cancer cells. Cancer Biol Ther. 2011; 12(10):924-38. PMC: 3280908. DOI: 10.4161/cbt.12.10.17780. View

Chen H, Chang Y, Chuang H, Tai W, Hwang J . Targeted therapy with fatty acid synthase inhibitors in a human prostate carcinoma LNCaP/tk-luc-bearing animal model. Prostate Cancer Prostatic Dis. 2012; 15(3):260-4. DOI: 10.1038/pcan.2012.15. View

Thomas K, Jacobson M . Defects in mitochondrial fission protein dynamin-related protein 1 are linked to apoptotic resistance and autophagy in a lung cancer model. PLoS One. 2012; 7(9):e45319. PMC: 3447926. DOI: 10.1371/journal.pone.0045319. View

10.

Ladiges W, Wanagat J, Preston B, Loeb L, Rabinovitch P . A mitochondrial view of aging, reactive oxygen species and metastatic cancer. Aging Cell. 2010; 9(4):462-5. DOI: 10.1111/j.1474-9726.2010.00579.x. View

11.

Elliott R, Jiang X, Head J . Mitochondria organelle transplantation: introduction of normal epithelial mitochondria into human cancer cells inhibits proliferation and increases drug sensitivity. Breast Cancer Res Treat. 2012; 136(2):347-54. DOI: 10.1007/s10549-012-2283-2. View

12.

Sotgia F, Whitaker-Menezes D, Martinez-Outschoorn U, Salem A, Tsirigos A, Lamb R . Mitochondria "fuel" breast cancer metabolism: fifteen markers of mitochondrial biogenesis label epithelial cancer cells, but are excluded from adjacent stromal cells. Cell Cycle. 2012; 11(23):4390-401. PMC: 3552922. DOI: 10.4161/cc.22777. View

13.

Yang W, Perillo W, Liou D, Marambaud P, Wang P . AMPK inhibitor compound C suppresses cell proliferation by induction of apoptosis and autophagy in human colorectal cancer cells. J Surg Oncol. 2012; 106(6):680-8. DOI: 10.1002/jso.23184. View

14.

Guo J, White E . Autophagy is required for mitochondrial function, lipid metabolism, growth, and fate of KRAS(G12D)-driven lung tumors. Autophagy. 2013; 9(10):1636-8. PMC: 5424446. DOI: 10.4161/auto.26123. View

15.

Salem A, Whitaker-Menezes D, Lin Z, Martinez-Outschoorn U, Tanowitz H, Al-Zoubi M . Two-compartment tumor metabolism: autophagy in the tumor microenvironment and oxidative mitochondrial metabolism (OXPHOS) in cancer cells. Cell Cycle. 2012; 11(13):2545-56. PMC: 3404881. DOI: 10.4161/cc.20920. View

16.

Das S, Das J, Samadder A, Boujedaini N, Khuda-Bukhsh A . Apigenin-induced apoptosis in A375 and A549 cells through selective action and dysfunction of mitochondria. Exp Biol Med (Maywood). 2013; 237(12):1433-48. DOI: 10.1258/ebm.2012.012148. View

17.

Kariya T, Wille L . Inhibition of fatty acid synthesis by RMI 14,514 (5-tetradecyloxy-2-furoic acid). Biochem Biophys Res Commun. 1978; 80(4):1022-4. DOI: 10.1016/0006-291x(78)91347-5. View

18.

Dong Y, Berners-Price S, Thorburn D, Antalis T, Dickinson J, Hurst T . Serine protease inhibition and mitochondrial dysfunction associated with cisplatin resistance in human tumor cell lines: targets for therapy. Biochem Pharmacol. 1997; 53(11):1673-82. DOI: 10.1016/s0006-2952(97)00015-4. View

19.

Baenke F, Peck B, Miess H, Schulze A . Hooked on fat: the role of lipid synthesis in cancer metabolism and tumour development. Dis Model Mech. 2013; 6(6):1353-63. PMC: 3820259. DOI: 10.1242/dmm.011338. View

20.

Ralph S, Rodriguez-Enriquez S, Neuzil J, Moreno-Sanchez R . Bioenergetic pathways in tumor mitochondria as targets for cancer therapy and the importance of the ROS-induced apoptotic trigger. Mol Aspects Med. 2009; 31(1):29-59. DOI: 10.1016/j.mam.2009.12.006. View