Antimicrobial Susceptibility and Molecular Characterization of Macrolide Resistance of Mycoplasma Bovis Isolates from Multiple Provinces in China

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2015 Sep 9

PMID 26346744

Citations 14

Authors

Ling-Cong Kong

Duo Gao

Bo-Yan Jia

Zi Wang

Yun-Hang Gao

Zhi-Hua Pei

Shu-Ming Liu

Jiu-Qing Xin

Hong-Xia Ma

Affiliations

Soon will be listed here.

Abstract

Mycoplasma bovis has spread widely throughout the world via animal movement and has become an important pathogen of bovine respiratory disease. However, the minimum inhibitory concentrations of antimicrobials for Mycoplasma bovis have not been studied in China. The objective of this study was to determine the prevalence and antibiotic resistance of Mycoplasma bovis isolated from young cattle with respiratory infection in China. Mycoplasma bovis was detected in 32/45 bovine respiratory infection outbreaks at beef farms in 8 provinces in China. The isolates were susceptible or had medium sensitivity to ciprofloxacin, enrofloxacin and doxycycline, but were frequently resistant to macrolides (13/32, 41%). An A2058G (Escherichia coli Numbering) mutation located in the rrnA operon in domain V of 23S rRNA was observed in strains that were resistant to macrolides. This single mutations at the rrnA operon in domain V of 23S rRNA may play an important role in the resistance of Mycoplasma bovis strains to macrolides.

Citing Articles

Exploring Metal Ions as Potential Antimicrobial Agents to Combat Future Drug Resistance in .

Alkhallawi M, Mohammed M, Hemmatzadeh F, Petrovski K Microorganisms. 2025; 13(1).

PMID: 39858937 PMC: 11767636. DOI: 10.3390/microorganisms13010169.

Unravelling Antimicrobial Resistance in : Genetic Mechanisms and Future Directions.

Jafari Jozani R, Al Khallawi M, Trott D, Petrovski K, Low W, Hemmatzadeh F Vet Sci. 2024; 11(11).

PMID: 39591316 PMC: 11598952. DOI: 10.3390/vetsci11110542.

A Set of Multiresistant Isolates of Subtype ST-1 with a Variable Susceptibility to Quinolones Are Also Circulating in Spain.

Corrales J, Sanchez A, Hernandez X, Amores-Iniesta J, Esnal A, de la Fe C Pathogens. 2024; 13(4).

PMID: 38668284 PMC: 11053527. DOI: 10.3390/pathogens13040329.

Toward a Method for Harmonized Susceptibility Testing of Mycoplasma bovis by Broth Microdilution.

Gutgemann F, Muller A, Churin Y, Kumm F, Braun A, Yue M J Clin Microbiol. 2023; 61(8):e0190522.

PMID: 37439667 PMC: 10446863. DOI: 10.1128/jcm.01905-22.

Transcriptomic Changes and Gene Function Analysis in with Different Levels of Resistance to Enrofloxacin.

Li X, Qi Y, Xue J, Xu G, Xu Y, Li X Vet Sci. 2023; 10(4).

PMID: 37104412 PMC: 10143902. DOI: 10.3390/vetsci10040257.

References

Shin S, Yoo M, Jeong J, Kim Y, Chung J, Huh M . Molecular cloning of the gyrA gene and characterization of its mutation in clinical isolates of quinolone-resistant Edwardsiella tarda. Dis Aquat Organ. 2006; 67(3):259-66. DOI: 10.3354/dao067259. View

Rosenbusch R, Kinyon J, Apley M, Funk N, Smith S, Hoffman L . In vitro antimicrobial inhibition profiles of Mycoplasma bovis isolates recovered from various regions of the United States from 2002 to 2003. J Vet Diagn Invest. 2005; 17(5):436-41. DOI: 10.1177/104063870501700505. View

Spigaglia P, Barbanti F, Louie T, Barbut F, Mastrantonio P . Molecular analysis of the gyrA and gyrB quinolone resistance-determining regions of fluoroquinolone-resistant Clostridium difficile mutants selected in vitro. Antimicrob Agents Chemother. 2009; 53(6):2463-8. PMC: 2687229. DOI: 10.1128/AAC.01252-08. View

Caswell J, Bateman K, Cai H, Castillo-Alcala F . Mycoplasma bovis in respiratory disease of feedlot cattle. Vet Clin North Am Food Anim Pract. 2010; 26(2):365-79. DOI: 10.1016/j.cvfa.2010.03.003. View

Bebear C, Pereyre S, Peuchant O . Mycoplasma pneumoniae: susceptibility and resistance to antibiotics. Future Microbiol. 2011; 6(4):423-31. DOI: 10.2217/fmb.11.18. View

Soehnlen M, Kunze M, Karunathilake K, Henwood B, Kariyawasam S, Wolfgang D . In vitro antimicrobial inhibition of Mycoplasma bovis isolates submitted to the Pennsylvania Animal Diagnostic Laboratory using flow cytometry and a broth microdilution method. J Vet Diagn Invest. 2011; 23(3):547-51. DOI: 10.1177/1040638711404155. View

Brenner Michael G, Eidam C, Kadlec K, Meyer K, Sweeney M, Murray R . Increased MICs of gamithromycin and tildipirosin in the presence of the genes erm(42) and msr(E)-mph(E) for bovine Pasteurella multocida and Mannheimia haemolytica. J Antimicrob Chemother. 2012; 67(6):1555-7. DOI: 10.1093/jac/dks076. View

Sato T, Okubo T, Usui M, Higuchi H, Tamura Y . Amino acid substitutions in GyrA and ParC are associated with fluoroquinolone resistance in Mycoplasma bovis isolates from Japanese dairy calves. J Vet Med Sci. 2013; 75(8):1063-5. DOI: 10.1292/jvms.12-0508. View

Lerner U, Amram E, Ayling R, Mikula I, Gerchman I, Harrus S . Acquired resistance to the 16-membered macrolides tylosin and tilmicosin by Mycoplasma bovis. Vet Microbiol. 2014; 168(2-4):365-71. DOI: 10.1016/j.vetmic.2013.11.033. View

10.

Gautier-Bouchardon A, Ferre S, Le Grand D, Paoli A, Gay E, Poumarat F . Overall decrease in the susceptibility of Mycoplasma bovis to antimicrobials over the past 30 years in France. PLoS One. 2014; 9(2):e87672. PMC: 3913625. DOI: 10.1371/journal.pone.0087672. View

11.

Olsen A, Warrass R, Douthwaite S . Macrolide resistance conferred by rRNA mutations in field isolates of Mannheimia haemolytica and Pasteurella multocida. J Antimicrob Chemother. 2014; 70(2):420-3. DOI: 10.1093/jac/dku385. View

12.

Kong L, Gao D, Gao Y, Liu S, Ma H . Fluoroquinolone resistance mechanism of clinical isolates and selected mutants of Pasteurella multocida from bovine respiratory disease in China. J Vet Med Sci. 2015; 76(12):1655-7. PMC: 4300385. DOI: 10.1292/jvms.14-0240. View

13.

Lysnyansky I, Gerchman I, Flaminio B, Catania S . Decreased Susceptibility to Macrolide-Lincosamide in Mycoplasma synoviae Is Associated with Mutations in 23S Ribosomal RNA. Microb Drug Resist. 2015; 21(6):581-9. DOI: 10.1089/mdr.2014.0290. View

14.

Rifatbegovic M, Assuncao P, Poveda J, Pasic S . Isolation of Mycoplasma bovis from the respiratory tract of cattle in Bosnia and Herzegovina. Vet Rec. 2007; 160(14):484-5. DOI: 10.1136/vr.160.14.484. View

15.

HALE H, Helmboldt C, PLASTRIDGE W, STULA E . Bovine mastitis caused by a Mycoplasma species. Cornell Vet. 1962; 52:582-91. View

16.

Gerchman I, Levisohn S, Mikula I, Lysnyansky I . In vitro antimicrobial susceptibility of Mycoplasma bovis isolated in Israel from local and imported cattle. Vet Microbiol. 2009; 137(3-4):268-75. DOI: 10.1016/j.vetmic.2009.01.028. View