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Evaluation of Housekeeping Genes As References for Quantitative Real-time PCR Analysis of Gene Expression in the Murrel Channa Striatus Under High-temperature Stress

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Specialty Biochemistry
Date 2015 Sep 8
PMID 26343884
Citations 11
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Abstract

Quantitative real-time polymerase chain reaction is the most advanced method of quantifying gene expression studies; however, the significance of the obtained results strongly depends on the normalization of the data to compensate for differences between the samples. In the present study, expression analysis of six different constitutively expressed genes viz. 18S ribosomal RNA, glyceraldehyde-3-phosphate dehydrogenase (gapdh), beta actin (βactin), ribosomal binding protein L13, tubulin and TATA-box-binding protein (tbp) were carried out to test their efficacy as reference genes in three different tissues, namely liver, gill and muscle of murrel Channa striatus exposed to high temperature for variable time periods. The stability and suitability of the genes were determined by using bioinformatic tools: GeNorm, NormFinder and BestKeeper. Based on the results, tub/βactin could be used as the reference genes for liver and gill tissues and βactin/gapdh could be the reference genes for muscle tissues in Channa striatus under both short- and long-term thermal stress.

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References
1.
Feng L, Yu Q, Li X, Ning X, Wang J, Zou J . Identification of reference genes for qRT-PCR analysis in Yesso scallop Patinopecten yessoensis. PLoS One. 2013; 8(9):e75609. PMC: 3777977. DOI: 10.1371/journal.pone.0075609. View

2.
Aursnes I, Rishovd A, Karlsen H, Gjoen T . Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress. BMC Res Notes. 2011; 4:104. PMC: 3080820. DOI: 10.1186/1756-0500-4-104. View

3.
Zhang B, Sun L, Xiao Z, Hu Y . Quantitative real time RT-PCR study of pathogen-induced gene expression in rock bream (Oplegnathus fasciatus): internal controls for data normalization. Mar Genomics. 2014; 15:75-84. DOI: 10.1016/j.margen.2014.03.001. View

4.
Urbatzka R, Galante-Oliveira S, Rocha E, Castro L, Cunha I . Normalization strategies for gene expression studies by real-time PCR in a marine fish species, Scophthalmus maximus. Mar Genomics. 2013; 10:17-25. DOI: 10.1016/j.margen.2013.02.001. View

5.
Small B, Murdock C, Bilodeau-Bourgeois A, Peterson B, Waldbieser G . Stability of reference genes for real-time PCR analyses in channel catfish (Ictalurus punctatus) tissues under varying physiological conditions. Comp Biochem Physiol B Biochem Mol Biol. 2008; 151(3):296-304. DOI: 10.1016/j.cbpb.2008.07.010. View